1 | %---------------------------------------------------------------------- |
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2 | % --- substract background to the images: rank the images by luminosity at each point and substracts to the images |
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3 | %---------------------------------------------------------------------- |
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4 | % Method: |
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5 | %calculate the background image by sorting the luminosity of each point |
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6 | % over a sliding sub-sequence of 'nbaver_ima' images. |
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7 | % The luminosity value of rank 'rank' is selected as the |
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8 | % 'background'. rank=nbimages/2 gives the median value. Smaller values are appropriate |
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9 | % for a dense set of particles. The extrem value rank=1 gives the true minimum |
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10 | % luminosity, but it can be polluted by noise. |
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11 | % Organization of image indices: |
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12 | % The program is working on a series of images, labelled by two indices i and j, given |
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13 | % by the input matlab vectors num_i1 and num_j1 respectively. In the list, j is the fastest increasing index. |
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14 | % The processing can be done in slices (number nbslice), with bursts of |
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15 | % nbfield2 successive images for a given slice (mode 'multilevel') |
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16 | % In the mode 'volume', nbfield2=1 (1 image at each level), and |
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17 | % nbslice= |
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18 | %INPUT: |
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19 | % num_i1: matrix of image indices i |
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20 | % num_j1: matrix of image indices j, must be the same size as num_i1 |
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21 | % num_i2 and num_j2: not used for a function acting on images |
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22 | % Series: matlab structure containing parameters, as defined by the interface UVMAT/series |
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23 | % Series.RootPath: path to the image series |
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24 | % Series.RootFile: root file name |
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25 | % Series.FileExt: image file extension |
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26 | % Series.NomType: nomenclature type for file indexing |
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27 | % Series.NbSlice: %number of slices defined on the interface |
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28 | |
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29 | function GUI_input=sub_background (num_i1,num_i2,num_j1,num_j2,Series) |
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30 | |
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31 | %------------------------------------------------------------------------ |
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32 | %requests for the visibility of input windows in the GUI series (activated directly by the selection in the menu ACTION) |
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33 | if ~exist('num_i1','var') |
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34 | GUI_input={'RootPath';'on';... |
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35 | 'SubDir';'off';... % subdirectory of derived files (PIV fields), ('on' by default) |
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36 | 'RootFile';'on';... %root input file name ('on' by default) |
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37 | 'FileExt';'on';... %inputf file extension ('on' by default) |
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38 | 'NomType';'on';...%type of file indexing ('on' by default) |
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39 | 'NbSlice';'on'; ...%nbre of slices ('off' by default) |
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40 | %'VelTypeMenu';'on';...% menu for selecting the velocity type (civ1,..)('off' by default) |
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41 | %'FieldMenu';'on';...% menu for selecting the velocity field (s) in the input file ('off' by default) |
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42 | %'VelTypeMenu_1';'on';...% menu for selecting the velocity type (civ1,..)('off' by default) |
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43 | %'FieldMenu_1';'on';...% menu for selecting the velocity field (s) in the input file ('off' by default) |
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44 | %'CoordType';...%can use a transform function |
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45 | %'GetObject';...;%can use projection object |
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46 | %'GetMask';...;%can use mask option |
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47 | 'PARAMETER';'NbSliding';... |
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48 | 'PARAMETER';'VolumeScan';... |
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49 | 'PARAMETER';'RankBrightness';... |
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50 | ''}; |
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51 | return %exit the function |
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52 | end |
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53 | |
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54 | %---------------------------------------------------------------- |
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55 | % initiate the waitbar |
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56 | hseries=guidata(Series.hseries);%handles of the GUI series |
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57 | WaitbarPos=get(hseries.waitbar_frame,'Position'); |
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58 | %----------------------------------------------------------------- |
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59 | if iscell(Series.RootPath) |
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60 | msgbox_uvmat('ERROR','This function use only one input image series') |
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61 | return |
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62 | end |
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63 | |
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64 | %determine input image type |
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65 | FileType=[];%default |
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66 | MovieObject=[]; |
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67 | if isequal(lower(FileExt),'.avi') |
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68 | hhh=which('mmreader'); |
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69 | if ~isequal(hhh,'')&& mmreader.isPlatformSupported() |
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70 | MovieObject=mmreader(fullfile(RootPath,[RootFile FileExt])); |
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71 | FileType='movie'; |
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72 | else |
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73 | FileType='avi'; |
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74 | end |
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75 | elseif isequal(lower(FileExt),'.vol') |
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76 | FileType='vol'; |
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77 | else |
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78 | form=imformats(FileExt(2:end)); |
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79 | if ~isempty(form)% if the extension corresponds to an image format recognized by Matlab |
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80 | if isequal(NomType,'*'); |
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81 | FileType='multimage'; |
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82 | else |
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83 | FileType='image'; |
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84 | end |
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85 | end |
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86 | end |
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87 | if isempty(FileType) |
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88 | msgbox_uvmat('ERROR',['invalid file extension ' FileExt ': this function only accepts image or movie input']) |
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89 | return |
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90 | end |
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91 | |
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92 | nbslice_i=Series.NbSlice; %number of slices |
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93 | siz=size(num_i1); |
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94 | nbaver_init=23;%approximate number of images used for the sliding background: to be adjusted later to include an integer number of bursts |
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95 | |
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96 | %adjust the proposed number of images in the sliding average to include an integer number of bursts |
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97 | if siz(2)~=1 |
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98 | nbaver=floor(nbaver_init/siz(1)); % number of bursts used for the sliding background, |
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99 | if isequal(floor(nbaver/2),nbaver) |
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100 | nbaver=nbaver+1;%put the number of burst to an odd number (so the middle burst is defined) |
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101 | end |
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102 | nbaver_init=nbaver*siz(1);%propose by default an integer number of bursts |
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103 | end |
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104 | |
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105 | filebase=fullfile(Series.RootPath,Series.RootFile); |
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106 | dir_images=Series.RootPath; |
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107 | nom_type=Series.NomType; |
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108 | |
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109 | %create dir of the new images |
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110 | [dir_images,namebase]=fileparts(filebase); |
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111 | [path,subdir_ima]=fileparts(dir_images); |
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112 | curdir=pwd; |
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113 | cd(path); |
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114 | mkdir([subdir_ima '_b']); |
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115 | cd(curdir); |
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116 | filebase_b=fullfile(path,[subdir_ima '_b'],namebase); |
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117 | |
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118 | prompt = {'Number of images for the sliding background';'The number of positions (laser slices)';'volume scan mode (Yes/No)';... |
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119 | 'the luminosity rank chosen to define the background (0.1=for dense particle seeding, 0.5 (median) for sparse particles'}; |
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120 | dlg_title = ['get (slice by slice) a sliding background and substract to each image, result in subdir ' subdir_ima '_b']; |
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121 | num_lines= 3; |
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122 | def = { num2str(nbaver_init);num2str(nbslice_i);'No';'0.1'}; |
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123 | answer = inputdlg(prompt,dlg_title,num_lines,def); |
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124 | set(hseries.ParamVal,'String',answer([1 [3:4]])) |
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125 | set(hseries.ParamVal,'Visible','on') |
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126 | |
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127 | nbaver_ima=str2num(answer{1});%number of images for the sliding background |
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128 | nbaver=ceil(nbaver_ima/siz(1))%number of bursts for the sliding background |
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129 | if isequal(floor(nbaver/2),nbaver) |
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130 | nbaver=nbaver+1%put the number of burst to an odd number (so the middle burst is defined) |
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131 | end |
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132 | % if isequal(nbaver,round(nbaver)) |
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133 | step=siz(1);%case of bursts: the sliding background is shifted by one burst |
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134 | % else |
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135 | % step=1; |
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136 | % end |
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137 | vol_test=answer{3}; |
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138 | if isequal(vol_test,'Yes') |
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139 | nbfield2=1;%case of volume: no consecutive series at a given level |
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140 | nbslice_i=siz(1);%number of slices |
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141 | else |
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142 | nbfield2=siz(1); %nb of consecutive images at each level(burst) |
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143 | if siz(2)>1 |
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144 | nbslice_i=str2num(answer{2})/(num_i1(1,2)-num_i1(1,1));% number of slices |
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145 | else |
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146 | nbslice_i=1; |
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147 | end |
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148 | if ~isequal(floor(nbslice_i),nbslice_i) |
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149 | msgbox_uvmat('ERROR','the number of slices must be a multiple of the i increment') |
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150 | return |
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151 | end |
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152 | end |
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153 | % nbaver=floor(nbaver_init/nbfield2); % number of bursts used for the sliding background, |
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154 | % if isequal(floor(nbaver/2),nbaver) |
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155 | % nbaver=nbaver+1;%put the number of burst to an odd number |
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156 | % end |
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157 | %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% |
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158 | %nbaver_ima=nbaver*nbfield2;% adjust the number of sliding images A REMETRE |
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159 | % if ~isequal(nbaver_ima,nbaver_init) |
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160 | % hwarn=warndlg(['number of images in the sliding average adjusted to ' num2str(nbaver_ima)]); |
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161 | % set(hwarn,'Units','normalized') |
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162 | % set(hwarn,'Position',[0.3 0.3 0.4 0.1]) |
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163 | % end |
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164 | %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% |
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165 | rank=floor(str2num(answer{4})*nbaver_ima); |
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166 | if rank==0 |
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167 | rank=1;%rank selected in the sorted image series |
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168 | end |
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169 | lengthtot=siz(1)*siz(2); |
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170 | nbfield=floor(lengthtot/(nbfield2*nbslice_i));%total number of i indexes (adjusted to an integer number of slices) |
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171 | nbfield_slice=nbfield*nbfield2;% number of fields per slice |
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172 | % test_plot=isequal(answer{5},'Yes'); %=1 to display background images |
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173 | if nbaver_ima > nbfield*nbfield2 |
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174 | errordlg('number of images in a slice smaller than the proposed number of images for the sliding average') |
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175 | return |
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176 | end |
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177 | nbfirst=(ceil(nbaver/2))*step; |
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178 | if nbfirst>nbaver_ima |
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179 | nbfirst=ceil(nbaver_ima/2) |
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180 | step=1; |
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181 | nbaver=nbaver_ima; |
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182 | end |
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183 | |
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184 | %copy the xml file |
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185 | if exist([filebase '.xml'],'file') |
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186 | copyfile([filebase '.xml'],[filebase_b '.xml']);% copy the .civ file |
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187 | t=xmltree([filebase_b '.xml']); |
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188 | |
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189 | %update information on the first image name in the series |
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190 | uid_Heading=find(t,'ImaDoc/Heading'); |
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191 | if isempty(uid_Heading) |
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192 | [t,uid_Heading]=add(t,1,'element','Heading'); |
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193 | end |
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194 | uid_ImageName=find(t,'ImaDoc/Heading/ImageName'); |
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195 | ImageName=name_generator(filebase_b,num_i1(1),num_j1(1),'.png',Series.NomType); |
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196 | [pth,ImageName]=fileparts(ImageName); |
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197 | ImageName=[ImageName '.png'] |
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198 | if isempty(uid_ImageName) |
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199 | [t,uid_ImageName]=add(t,uid_Heading,'element','ImageName'); |
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200 | end |
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201 | uid_value=children(t,uid_ImageName); |
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202 | if isempty(uid_value) |
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203 | t=add(t,uid_ImageName,'chardata',ImageName)%indicate name of the first image, with ;png extension |
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204 | else |
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205 | t=set(t,uid_value(1),'value',ImageName)%indicate name of the first image, with ;png extension |
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206 | end |
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207 | |
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208 | %add information about image transform |
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209 | [t,new_uid]=add(t,1,'element','ImageTransform'); |
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210 | [t,NameFunction_uid]=add(t,new_uid,'element','NameFunction'); |
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211 | [t]=add(t,NameFunction_uid,'chardata','sub_background'); |
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212 | [t,NbSlice_uid]=add(t,new_uid,'element','NbSlice'); |
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213 | [t]=add(t,new_uid,'chardata',num2str(nbslice_i)); |
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214 | [t,NbSlidingImages_uid]=add(t,new_uid,'element','NbSlidingImages'); |
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215 | [t]=add(t,NbSlidingImages_uid,'chardata',num2str(nbaver)); |
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216 | [t,LuminosityRank_uid]=add(t,new_uid,'element','RankBackground'); |
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217 | [t]=add(t,LuminosityRank_uid,'chardata',num2str(rank));% luminosity rank almong the nbaver sliding images |
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218 | save(t,[filebase_b '.xml']) |
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219 | elseif exist([filebase '.civ'],'file') |
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220 | copyfile([filebase '.civ'],[filebase_b '.civ']);% copy the .civ file |
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221 | end |
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222 | %copy the mask |
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223 | if exist([filebase '_1mask_1'],'file') |
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224 | copyfile([filebase '_1mask_1'],[filebase_b '_1mask_1']);% copy the mask file |
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225 | end |
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226 | |
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227 | %MAIN LOOP ON SLICES |
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228 | for islice=1:nbslice_i |
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229 | %select the series of image indices at the level islice |
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230 | for ifield=1:nbfield |
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231 | for iburst=1:nbfield2 |
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232 | indselect(iburst,ifield)=((ifield-1)*nbslice_i+(islice-1))*nbfield2+iburst; |
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233 | end |
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234 | end |
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235 | %read the first series of nbaver_ima images and sort by luminosity at each pixel |
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236 | for ifield = 1:nbaver_ima |
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237 | ifile=indselect(ifield); |
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238 | filename=name_generator(filebase,num_i1(ifile),num_j1(ifile),Series.FileExt,Series.NomType); |
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239 | Aread=read_image(filename,FileType,num_i1(ifile),movieobject); |
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240 | Ak(:,:,ifield)=Aread; |
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241 | end |
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242 | Asort=sort(Ak,3);%sort the luminosity of images at each point |
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243 | B=Asort(:,:,rank);%background image |
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244 | % |
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245 | % namemean=name_generator([filebase '_back'],islice,[],'.png','_i');% makes the file name |
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246 | % imwrite(B,namemean,'BitDepth',16); % save the first background image |
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247 | % ['background image for slice ' num2str(islice) ' saved in ' namemean] |
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248 | %substract the background from each of the first images and save the new images |
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249 | % for ifield=1:floor(nbaver_ima/2)+1 |
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250 | 'first background image will be substracted' |
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251 | |
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252 | for ifield=1:nbfirst |
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253 | Acor=double(Ak(:,:,ifield))-double(B);%substract background to the current image |
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254 | Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor |
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255 | C=uint16(Acor);% set to integer 16 bits |
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256 | ifile=indselect(ifield); |
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257 | newname=name_generator(filebase_b,num_i1(ifile),num_j1(ifile),'.png',nom_type)% makes the new file name |
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258 | imwrite(C,newname,'BitDepth',16); % save the new image |
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259 | end |
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260 | %repeat the operation on a sliding series of nbaver*nbfield2 images |
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261 | 'sliding background image will be substracted' |
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262 | if nbfield_slice > nbaver_ima |
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263 | % for ifield = floor(nbaver_ima/2)+2:step:nbfield_slice-floor(nbaver_ima/2) |
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264 | for ifield = step*ceil(nbaver/2)+1:step:nbfield_slice-step*floor(nbaver/2) |
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265 | stopstate=get(hseries.RUN,'BusyAction'); |
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266 | if isequal(stopstate,'queue')% enable STOP command |
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267 | update_waitbar(hseries.waitbar,WaitbarPos,(ifield+(islice-1)*nbfield_slice)/(nbfield_slice*nbslice_i)) |
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268 | (ifield+(islice-1)*nbfield_slice)/(nbfield_slice*nbslice_i) |
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269 | Ak(:,:,[1:nbaver_ima-step])=Ak(:,:,[1+step:nbaver_ima]);% shift the current image series by one burst (step) |
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270 | %incorporate next burst in the current image series |
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271 | for iburst=1:step |
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272 | ifile=indselect(ifield+step*floor(nbaver/2)+iburst-1); |
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273 | filename=name_generator(filebase,num_i1(ifile),num_j1(ifile),Series.FileExt,Series.NomType); |
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274 | Aread=read_image(filename,FileType,num_i1(ifile),movieobject); |
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275 | Ak(:,:,nbaver_ima-step+iburst)=Aread; |
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276 | end |
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277 | Asort=sort(Ak,3);%sort the new current image series by luminosity |
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278 | B=Asort(:,:,rank);%current background image |
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279 | for iburst=1:step |
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280 | % Acor=double(Ak(:,:,floor(nbaver_ima/2)+iburst-1))-double(B); |
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281 | index=step*floor(nbaver/2)+iburst; |
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282 | Acor=double(Ak(:,:,index))-double(B); |
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283 | Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor |
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284 | C=uint16(Acor); |
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285 | ifile=indselect(ifield+iburst-1); |
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286 | [newname]=... |
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287 | name_generator(filebase_b,num_i1(ifile),num_j1(ifile),'.png',Series.NomType) |
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288 | % newname=name_generator(filebase_b,num_i1(indselect(ifield+iburst-1)),num_j1(indselect(ifield+iburst-1)),'.png',nom_type)% makes the new file name |
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289 | imwrite(C,newname,'BitDepth',16); % save the new image |
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290 | end |
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291 | else |
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292 | return |
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293 | end |
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294 | end |
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295 | end |
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296 | |
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297 | %substract the background from the last images |
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298 | % for ifield=nbfield_slice-floor(nbaver_ima/2)+1:nbfield_slice |
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299 | 'last background image will be substracted' |
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300 | ifield=nbfield_slice-(step*ceil(nbaver/2))+1:nbfield_slice; |
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301 | for ifield=nbfield_slice-(step*floor(nbaver/2))+1:nbfield_slice |
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302 | index=ifield-nbfield_slice+step*(2*floor(nbaver/2)+1); |
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303 | Acor=double(Ak(:,:,index))-double(B); |
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304 | Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor |
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305 | C=uint16(Acor); |
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306 | ifile=indselect(ifield); |
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307 | newname=name_generator(filebase_b,num_i1(ifile),num_j1(ifile),'.png',nom_type)% makes the new file name |
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308 | imwrite(C,newname,'BitDepth',16); % save the new image |
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309 | end |
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310 | end |
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311 | |
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312 | %finish the waitbar |
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313 | update_waitbar(hseries.waitbar,WaitbarPos,1) |
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314 | |
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315 | |
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316 | %------------------------------------------------------------------------ |
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317 | %--read images and convert them to the uint16 format used for PIV |
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318 | function A=read_image(filename,type_ima,num,movieobject) |
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319 | %------------------------------------------------------------------------ |
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320 | %num is the view number needed for an avi movie |
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321 | switch type_ima |
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322 | case 'movie' |
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323 | A=read(movieobject,num); |
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324 | case 'avi' |
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325 | mov=aviread(filename,num); |
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326 | A=frame2im(mov(1)); |
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327 | case 'multimage' |
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328 | A=imread(filename,num); |
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329 | case 'image' |
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330 | A=imread(filename); |
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331 | end |
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332 | siz=size(A); |
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333 | if length(siz)==3;%color images |
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334 | A=sum(double(A),3); |
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335 | end |
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336 | |
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