source: trunk/src/series/sub_background.m @ 457

Last change on this file since 457 was 457, checked in by sommeria, 12 years ago

merg_i_j transformed to the new standards.
Various improvements

File size: 21.1 KB
Line 
1%'sub_background': substract a sliding background to an image series
2% This is an example of action on a series of input images
3%------------------------------------------------------------------------
4% Method:
5    %calculate the background image by sorting the luminosity of each point
6    % over a sliding sub-sequence of 'nbaver_ima' images.
7    % The luminosity value of rank 'rank' is selected as the
8    % 'background'. rank=nbimages/2 gives the median value.  Smaller values are appropriate
9    % for a dense set of particles. The extrem value rank=1 gives the true minimum
10    % luminosity, but it can be polluted by noise.
11% Organization of image indices:
12    % The program is working on a series of images,
13    % The processing can be done over groups of nbfield2 consecutive files in slices (parameter NbSlice)
14    % In the mode 'volume', nbfield2=1 (1 image at each level)and NbSlice (=nbfield_j)
15    % Else nbfield2=nbfield_j =nbre of images in a burst (j index)
16   
17% function GUI_config=sub_background(Param)
18%
19%%%%%%%%%%% GENERAL TO ALL SERIES ACTION FCTS %%%%%%%%%%%%%%%%%%%%%%%%%%%
20%
21% This function is used in four modes by the GUI series:
22%           1) config GUI: with no input argument, the function determine the suitable GUI configuration
23%           2) interactive input: the function is used to interactively introduce input parameters, and then stops
24%           3) RUN: the function itself runs, when an appropriate input  structure Param has been introduced.
25%           4) BATCH: the function itself proceeds in BATCH mode, using an xml file 'Param' as input.
26%
27%OUTPUT
28% GUI_series_config=list of options in the GUI series.fig needed for the function
29%
30%INPUT:
31% In run mode, the input parameters are given as a Matlab structure Param copied from the GUI series.
32% In batch mode, Param is the name of the corresponding xml file containing the same information
33% In the absence of input (as activated when the current Action is selected
34% in series), the function ouput GUI_series_config set the activation of the needed GUI elements
35%
36% Param contains the elements:(use the menu bar command 'export/GUI config' in series to see the current structure Param)
37%    .InputTable: cell of input file names, (several lines for multiple input)
38%                      each line decomposed as {RootPath,SubDir,Rootfile,NomType,Extension}
39%    .OutputSubDir: name of the subdirectory for data outputs
40%    .OutputDir: directory for data outputs, including path
41%    .Action: .ActionName: name of the current activated function
42%             .ActionPath:   path of the current activated function
43%    .IndexRange: set the file or frame indices on which the action must be performed
44%    .FieldTransform: .TransformName: name of the selected transform function
45%                     .TransformPath:   path  of the selected transform function
46%                     .TransformHandle: corresponding function handle
47%    .InputFields: sub structure describing the input fields withfields
48%              .FieldName: name of the field
49%              .VelType: velocity type
50%              .FieldName_1: name of the second field in case of two input series
51%              .VelType_1: velocity type of the second field in case of two input series
52%    .ProjObject: %sub structure describing a projection object (read from ancillary GUI set_object)
53%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
54
55   
56function GUI_config=sub_background (Param)
57
58%% set the input elements needed on the GUI series when the action is selected in the menu ActionName
59if ~exist('Param','var') % case with no input parameter
60    GUI_config={'NbViewMax';1;...% max nbre of input file series (default='' , no limitation)
61        'AllowInputSort';'off';...% allow alphabetic sorting of the list of input files (options 'off'/'on', 'off' by default)
62        'WholeIndexRange';'on';...% prescribes the file index ranges from min to max (options 'off'/'on', 'off' by default)
63        'NbSlice';'on'; ...%nbre of slices ('off' by default)
64        'VelType';'off';...% menu for selecting the velocity type (options 'off'/'one'/'two',  'off' by default)
65        'FieldName';'off';...% menu for selecting the field (s) in the input file(options 'off'/'one'/'two', 'off' by default)
66        'FieldTransform'; 'off';...%can use a transform function
67        'ProjObject';'off';...%can use projection object(option 'off'/'on',
68        'Mask';'off';...%can use mask option   (option 'off'/'on', 'off' by default)
69        'OutputDirExt';'.sback';...%set the output dir extension
70               ''};
71        return
72end
73
74%%%%%%%%%%%% STANDARD PART (DO NOT EDIT) %%%%%%%%%%%%
75%% select different modes,  RUN, parameter input, BATCH
76% BATCH  case: read the xml file for batch case
77if ischar(Param)
78    if strcmp(Param,'input?')
79        checkrun=1;% will inly search input parameters (preparation of BATCH mode)
80    else
81        Param=xml2struct(Param);
82        checkrun=0;
83    end
84% RUN case: parameters introduced as the input structure Param
85else
86    hseries=guidata(Param.hseries);%handles of the GUI series
87    WaitbarPos=get(hseries.waitbar_frame,'Position');%position of the waitbar on the GUI series
88    checkrun=2; % indicate the RUN option is used
89end
90
91%% root input file(s) and type
92RootPath=Param.InputTable(:,1);
93RootFile=Param.InputTable(:,3);
94SubDir=Param.InputTable(:,2);
95NomType=Param.InputTable(:,4);
96FileExt=Param.InputTable(:,5);
97
98% get the set of input file names (cell array filecell), and the lists of
99% input file or frame indices i1_series,i2_series,j1_series,j2_series
100[filecell,i1_series,i2_series,j1_series,j2_series]=get_file_series(Param);
101% filecell{iview,fileindex}: cell array representing the list of file names
102%        iview: line in the table corresponding to a given file series
103%        fileindex: file index within  the file series,
104% i1_series(iview,ref_j,ref_i)... are the corresponding arrays of indices i1,i2,j1,j2, depending on the input line iview and the two reference indices ref_i,ref_j
105% i1_series(iview,fileindex) expresses the same indices as a 1D array in file indices
106% set of frame indices used for movie or multimage input
107% numbers of slices and file indices
108
109NbSlice=1;%default
110if isfield(Param.IndexRange,'NbSlice')&&~isempty(Param.IndexRange.NbSlice)
111    NbSlice=Param.IndexRange.NbSlice;
112end
113nbview=numel(i1_series);%number of input file series (lines in InputTable)
114nbfield_j=size(i1_series{1},1); %nb of fields for the j index (bursts or volume slices)
115nbfield_i=size(i1_series{1},2); %nb of fields for the i index
116nbfield=nbfield_j*nbfield_i; %total number of fields
117nbfield_i=floor(nbfield/NbSlice);%total number of  indexes in a slice (adjusted to an integer number of slices)
118nbfield=nbfield_i*NbSlice; %total number of fields after adjustement
119
120%determine the file type on each line from the first input file
121ImageTypeOptions={'image','multimage','mmreader','video'};%allowed input file types(images)
122
123[FileType{1},FileInfo{1},MovieObject{1}]=get_file_type(filecell{1,1});
124CheckImage{1}=~isempty(find(strcmp(FileType,ImageTypeOptions)));% =1 for images
125if ~isempty(j1_series{1})
126    frame_index{1}=j1_series{1};
127else
128    frame_index{1}=i1_series{1};
129end
130
131%% calibration data and timing: read the ImaDoc files
132%not relevant here
133
134%% check coincidence in time for several input file series
135%not relevant here
136
137%% coordinate transform or other user defined transform
138%not relevant here
139
140%%%%%%%%%%%% END STANDARD PART  %%%%%%%%%%%%
141 % EDIT FROM HERE
142
143 %% check the validity of  input file types
144if CheckImage{1}
145    FileExtOut='.png'; % write result as .png images for image inputs
146    if strcmp(lower(NomType{1}(end)),'a')
147        NomTypeOut=NomType{1};%case of letter appendix
148    else
149        NomTypeOut='_1_1';% caseof purely numerical indexing
150    end
151else
152    msgbox_uvmat('ERROR',['invalid file type input: ' FileType{1} ' not an image'])
153    return
154end
155
156%% Set field names and velocity types
157%not relevant here
158
159%% Initiate output fields
160%not relevant here
161 
162%%% SPECIFIC PART BEGINS HERE
163NbSlice=Param.IndexRange.NbSlice; %number of slices
164%siz=size(i1_series);
165nbaver_init=23;%approximate number of images used for the sliding background: to be adjusted later to include an integer number of bursts
166j1=[];%default
167
168%% adjust the proposed number of images in the sliding average to include an integer number of bursts
169if nbfield_i~=1
170    nbaver=floor(nbaver_init/nbfield_j); % number of bursts used for the sliding background,
171    if isequal(floor(nbaver/2),nbaver)
172        nbaver=nbaver+1;%put the number of burst to an odd number (so the middle burst is defined)
173    end
174    nbaver_init=nbaver*nbfield_j;%propose by default an integer number of bursts
175end
176
177%% input of specific parameters
178if checkrun %get specific parameters interactively
179    prompt = {'volume scan mode (Yes/No)';'Number of images for the sliding background (MUST FIT IN COMPUTER MEMORY)';...
180        'the luminosity rank chosen to define the background (0.1=for dense particle seeding, 0.5 (median) for sparse particles'};
181    dlg_title = ['get (slice by slice) a sliding background and substract to each image, result in subdir ' Param.OutputDir];
182    num_lines= 3;
183    def     = { 'No';num2str(nbaver_init);'0.1'};
184    answer = inputdlg(prompt,dlg_title,num_lines,def);
185   
186    %check input consistency
187    if strcmp(answer{1},'No') && ~isequal(NbSlice,1)
188        check=msgbox_uvmat('INPUT_Y-N',['confirm the multi-level splitting into ' num2str(NbSlice) ' slices']);
189        if ~strcmp(check,'Yes')
190            return
191        end
192    end
193    if strcmp(answer{1},'Yes')
194        step=1;
195    else
196        step=nbfield_j;%case of bursts: the sliding background is shifted by the length of one burst
197    end
198    nbaver_ima=str2num(answer{2});%number of images for the sliding background
199    nbaver=ceil(nbaver_ima/step);%number of bursts for the sliding background
200    if isequal(floor(nbaver/2),nbaver)
201        nbaver=nbaver+1;%set the number of bursts to an odd number (so the middle burst is defined)
202    end
203    nbaver_ima=nbaver*step;
204    if nbaver_ima > nbfield
205        msgbox_uvmat('ERROR','number of images in a slice smaller than the proposed number of images for the sliding average')
206        return
207    end
208    GUI_config.CheckVolume=strcmp(answer{1},'Yes');
209    GUI_config.SlidingSequenceSize=nbaver_ima;
210    GUI_config.BrightnessRankThreshold=str2num(answer{3});
211   
212    % apply the image rescaling function 'level' (avoid the blinking effects of bright particles)
213    answer=msgbox_uvmat('INPUT_Y-N','apply image rescaling function levels.m after sub_background');
214    GUI_config.CheckLevelTransform=strcmp(answer,'Yes');
215    if checkrun==2
216         return
217    end
218    %%%%%%%%%%%%%%%%%%%%%%  STOP HERE FOR PAMETER INPUT MODE  %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
219else
220    GUI_config=Param.Specific;
221    if isequal(GUI_config.CheckVolume,1)
222        step=1;
223    else
224        step=nbfield_j;%case of bursts: the sliding background is shifted by the length of one burst
225    end
226    nbaver_ima=GUI_config.SlidingSequenceSize;%number of images for the sliding background
227    nbaver=ceil(nbaver_ima/step);%number of bursts for the sliding background
228    if isequal(floor(nbaver/2),nbaver)
229        nbaver=nbaver+1;%set the number of bursts to an odd number (so the middle burst is defined)
230    end
231    nbaver_ima=nbaver*step;
232    if nbaver_ima > nbfield
233        msgbox_uvmat('ERROR','number of images in a slice smaller than the proposed number of images for the sliding average')
234        return
235    end
236end
237
238% calculate absolute brightness rank
239rank=floor(GUI_config.BrightnessRankThreshold*nbaver_ima);
240if rank==0
241    rank=1;%rank selected in the sorted image series
242end
243
244%% prealocate memory for the sliding background
245try
246    Afirst=read_image(filecell{1,1},FileType{1},MovieObject{1},frame_index{1}(1));
247    [npy,npx]=size(Afirst);
248    if strcmp(class(Afirst),'uint8') % case of 8bit images
249        Ak=zeros(npy,npx,nbaver_ima,'uint8'); %prealocate memory
250        Asort=zeros(npy,npx,nbaver_ima,'uint8'); %prealocate memory
251    else
252        Ak=zeros(npy,npx,nbaver_ima,'uint16'); %prealocate memory
253        Asort=zeros(npy,npx,nbaver_ima,'uint16'); %prealocate memory
254    end
255catch ME
256    msgbox_uvmat('ERROR',ME.message)
257    return
258end
259
260%% update the xml file
261% SubDirBase=regexprep(Param.InputTable{1,2},'\..*','');%take the root part of SubDir, before the first dot '.'
262% filexml=fullfile(RootPath{1},[SubDirBase '.xml']);
263% if ~exist(filexml,'file') && exist([fullfile(RootPath{1},SubDir{1},RootFile{1}) '.xml'],'file')% xml inside the image directory
264%     copyfile([filebase '.xml'],filexml);% copy the .xml file
265% end
266% if exist(filexml,'file')
267%     t=xmltree(filexml); 
268%     %update information on the first image name in the series
269%     uid_Heading=find(t,'ImaDoc/Heading');
270%     if isempty(uid_Heading)
271%         [t,uid_Heading]=add(t,1,'element','Heading');
272%     end
273%     uid_ImageName=find(t,'ImaDoc/Heading/ImageName');
274%     if ~isempty(j1_series{1})
275%         j1=j1_series{1}(1);
276%     end
277%     ImageName=fullfile_uvmat([dir_images term],'',RootFile{1},'.png',NomType,i1_series(1,1),[],j1);
278%     [pth,ImageName]=fileparts(ImageName);
279%     ImageName=[ImageName '.png'];
280%     if isempty(uid_ImageName)
281%         [t,uid_ImageName]=add(t,uid_Heading,'element','ImageName');
282%     end
283%     uid_value=children(t,uid_ImageName);
284%     if isempty(uid_value)
285%         t=add(t,uid_ImageName,'chardata',ImageName);%indicate  name of the first image, with ;png extension
286%     else
287%         t=set(t,uid_value(1),'value',ImageName);%indicate  name of the first image, with ;png extension
288%     end
289%     
290%     %add information about image transform
291%     [t,new_uid]=add(t,1,'element','ImageTransform');
292%     [t,NameFunction_uid]=add(t,new_uid,'element','NameFunction');
293%     [t]=add(t,NameFunction_uid,'chardata','sub_background');
294%     if GUI_config.CheckLevel
295%         [t,NameFunction_uid]=add(t,new_uid,'element','NameFunction');
296%         [t]=add(t,NameFunction_uid,'chardata','levels');
297%     end
298%     [t,NbSlice_uid]=add(t,new_uid,'element','NbSlice');
299%     [t]=add(t,new_uid,'chardata',num2str(NbSlice));
300%     [t,NbSlidingImages_uid]=add(t,new_uid,'element','NbSlidingImages');
301%     [t]=add(t,NbSlidingImages_uid,'chardata',num2str(nbaver));
302%     [t,LuminosityRank_uid]=add(t,new_uid,'element','RankBackground');
303%     [t]=add(t,LuminosityRank_uid,'chardata',num2str(rank));% luminosity rank almong the nbaver sliding images
304%     save(t,filexml)
305% end
306%copy the mask
307% if exist([filebase '_1mask_1'],'file')
308%     copyfile([filebase '_1mask_1'],[filebase_b '_1mask_1']);% copy the mask file
309% end
310
311%MAIN LOOP ON SLICES
312for islice=1:NbSlice
313    %% select the series of image indices at the level islice
314    indselect=islice:NbSlice*step:nbfield;% select file indices of the slice
315    for ifield=1:step-1
316        indselect=[indselect;indselect(end,:)+1];
317    end
318   
319    %% read the first series of nbaver_ima images and sort by luminosity at each pixel
320    for ifield = 1:nbaver_ima
321        ifile=indselect(ifield);
322        filename=filecell{1,ifile};
323        Aread=read_image(filename,FileType{1},MovieObject{1},frame_index{1}(ifile));
324        if ndims(Aread)==3;%color images
325            Aread=sum(double(Aread),3);% take the sum of color components
326        end
327        Ak(:,:,ifield)=Aread;
328    end
329    Asort=sort(Ak,3);%sort the luminosity of images at each point
330    B=Asort(:,:,rank);%background image
331    display( 'first background image will be substracted')
332    nbfirst=(ceil(nbaver/2))*step;
333    for ifield=1:nbfirst
334        Acor=double(Ak(:,:,ifield))-double(B);%substract background to the current image
335        Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
336        ifile=indselect(ifield);
337        if ~isempty(j1_series{1})
338            j1=j1_series{1}(ifile);
339        end
340        newname=fullfile_uvmat(RootPath{1},Param.OutputSubDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
341       
342        %write result file
343        if GUI_config.CheckLevelTransform
344            C=levels(Acor);
345            imwrite(C,newname,'BitDepth',8); % save the new image
346        else
347            if isequal(FileInfo{1}.BitDepth,16)
348                C=uint16(Acor);
349                imwrite(C,newname,'BitDepth',16); % save the new image
350            else
351                C=uint8(Acor);
352                imwrite(C,newname,'BitDepth',8); % save the new image
353            end
354        end
355        display([newname ' written'])
356    end
357   
358    %% repeat the operation on a sliding series of images
359    display('sliding background image will be substracted')
360    if nbfield_i > nbaver_ima
361        for ifield = step*ceil(nbaver/2)+1:step:nbfield_i-step*floor(nbaver/2)
362            if checkrun
363                stopstate=get(hseries.RUN,'BusyAction');
364                update_waitbar(hseries.waitbar_frame,WaitbarPos,(ifield+(islice-1)*nbfield_i)/(nbfield_i*NbSlice))
365            else
366                stopstate='queue';
367            end
368            if isequal(stopstate,'queue')% enable STOP command
369                Ak(:,:,1:nbaver_ima-step)=Ak(:,:,1+step:nbaver_ima);% shift the current image series by one burst (step)
370                %incorporate next burst in the current image series
371                for iburst=1:step
372                    ifile=indselect(ifield+step*floor(nbaver/2)+iburst-1);
373                    filename=fullfile_uvmat(RootPath{1},SubDir{1},RootFile{1},FileExt{1},NomType{1},i1_series{1}(ifile),[],j1_series{1}(ifile));
374                    Aread=read_image(filename,FileType{1},MovieObject{1},i1_series{1}(ifile));
375                    if ndims(Aread)==3;%color images
376                        Aread=sum(double(Aread),3);% take the sum of color components
377                    end
378                    Ak(:,:,nbaver_ima-step+iburst)=Aread;
379                end
380                Asort=sort(Ak,3);%sort the new current image series by luminosity
381                B=Asort(:,:,rank);%current background image
382                for iburst=1:step
383                    index=step*floor(nbaver/2)+iburst;
384                    Acor=double(Ak(:,:,index))-double(B);
385                    Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
386                    ifile=indselect(ifield+iburst-1);
387                    if ~isempty(j1_series{1})
388                        j1=j1_series{1}(ifile);
389                    end
390                    newname=fullfile_uvmat(RootPath{1},Param.OutputSubDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
391                    %write result file
392                    if GUI_config.CheckLevelTransform
393                        C=levels(Acor);
394                        imwrite(C,newname,'BitDepth',8); % save the new image
395                    else
396                        if isequal(FileInfo{1}.BitDepth,16)
397                            C=uint16(Acor);
398                            imwrite(C,newname,'BitDepth',16); % save the new image
399                        else
400                            C=uint8(Acor);
401                            imwrite(C,newname,'BitDepth',8); % save the new image
402                        end
403                    end
404                    display([newname ' written'])
405                   
406                end
407            else
408                return
409            end
410        end
411    end
412   
413    %% substract the background from the last images
414    display('last background image will be substracted')
415    ifield=nbfield_i-(step*ceil(nbaver/2))+1:nbfield_i;
416    for ifield=nbfield_i-(step*floor(nbaver/2))+1:nbfield_i
417        index=ifield-nbfield_i+step*(2*floor(nbaver/2)+1);
418        Acor=double(Ak(:,:,index))-double(B);
419        Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
420        ifile=indselect(ifield);
421        if ~isempty(j1_series{1})
422            j1=j1_series{1}(ifile);
423        end
424        newname=fullfile_uvmat(RootPath{1},Param.OutputSubDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
425       
426        %write result file
427        if GUI_config.CheckLevelTransform
428            C=levels(Acor);
429            imwrite(C,newname,'BitDepth',8); % save the new image
430        else
431            if isequal(FileInfo{1}.BitDepth,16)
432                C=uint16(Acor);
433                imwrite(C,newname,'BitDepth',16); % save the new image
434            else
435                C=uint8(Acor);
436                imwrite(C,newname,'BitDepth',8); % save the new image
437            end
438        end
439        display([newname ' written'])
440    end
441end
442
443%finish the waitbar
444if checkrun
445    update_waitbar(hseries.waitbar,WaitbarPos,1)
446end
447   
448
449function C=levels(A)
450%whos A;
451B=double(A(:,:,1));
452windowsize=round(min(size(B,1),size(B,2))/20);
453windowsize=floor(windowsize/2)*2+1;
454ix=1/2-windowsize/2:-1/2+windowsize/2;%
455%del=np/3;
456%fct=exp(-(ix/del).^2);
457fct2=cos(ix/(windowsize-1)/2*pi/2);
458%Mfiltre=(ones(5,5)/5^2);
459%Mfiltre=fct2';
460Mfiltre=fct2'*fct2;
461Mfiltre=Mfiltre/(sum(sum(Mfiltre)));
462
463C=filter2(Mfiltre,B);
464C(:,1:windowsize)=C(:,windowsize)*ones(1,windowsize);
465C(:,end-windowsize+1:end)=C(:,end-windowsize+1)*ones(1,windowsize);
466C(1:windowsize,:)=ones(windowsize,1)*C(windowsize,:);
467C(end-windowsize+1:end,:)=ones(windowsize,1)*C(end-windowsize,:);
468C=tanh(B./(2*C));
469[n,c]=hist(reshape(C,1,[]),100);
470% figure;plot(c,n);
471
472[m,i]=max(n);
473c_max=c(i);
474[dummy,index]=sort(abs(c-c(i)));
475n=n(index);
476c=c(index);
477i_select = find(cumsum(n)<0.95*sum(n));
478if isempty(i_select)
479    i_select = 1:length(c);
480end
481c_select=c(i_select);
482n_select=n(i_select);
483cmin=min(c_select);
484cmax=max(c_select);
485C=(C-cmin)/(cmax-cmin)*256;
486C=uint8(C);
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