source: trunk/src/series/sub_background.m @ 462

Last change on this file since 462 was 462, checked in by sommeria, 12 years ago

a few bug repairs and update in the series fcts

File size: 21.3 KB
Line 
1%'sub_background': substract a sliding background to an image series
2% This is an example of action on a series of input images
3%------------------------------------------------------------------------
4% Method:
5    %calculate the background image by sorting the luminosity of each point
6    % over a sliding sub-sequence of 'nbaver_ima' images.
7    % The luminosity value of rank 'rank' is selected as the
8    % 'background'. rank=nbimages/2 gives the median value.  Smaller values are appropriate
9    % for a dense set of particles. The extrem value rank=1 gives the true minimum
10    % luminosity, but it can be polluted by noise.
11% Organization of image indices:
12    % The program is working on a series of images,
13    % The processing can be done over groups of nbfield2 consecutive files in slices (parameter NbSlice)
14    % In the mode 'volume', nbfield2=1 (1 image at each level)and NbSlice (=nbfield_j)
15    % Else nbfield2=nbfield_j =nbre of images in a burst (j index)
16   
17% function GUI_config=sub_background(Param)
18%
19%%%%%%%%%%% GENERAL TO ALL SERIES ACTION FCTS %%%%%%%%%%%%%%%%%%%%%%%%%%%
20%
21% This function is used in four modes by the GUI series:
22%           1) config GUI: with no input argument, the function determine the suitable GUI configuration
23%           2) interactive input: the function is used to interactively introduce input parameters, and then stops
24%           3) RUN: the function itself runs, when an appropriate input  structure Param has been introduced.
25%           4) BATCH: the function itself proceeds in BATCH mode, using an xml file 'Param' as input.
26%
27%OUTPUT
28% GUI_series_config=list of options in the GUI series.fig needed for the function
29%
30%INPUT:
31% In run mode, the input parameters are given as a Matlab structure Param copied from the GUI series.
32% In batch mode, Param is the name of the corresponding xml file containing the same information
33% In the absence of input (as activated when the current Action is selected
34% in series), the function ouput GUI_series_config set the activation of the needed GUI elements
35%
36% Param contains the elements:(use the menu bar command 'export/GUI config' in series to see the current structure Param)
37%    .InputTable: cell of input file names, (several lines for multiple input)
38%                      each line decomposed as {RootPath,SubDir,Rootfile,NomType,Extension}
39%    .OutputSubDir: name of the subdirectory for data outputs
40%    .OutputDir: directory for data outputs, including path
41%    .Action: .ActionName: name of the current activated function
42%             .ActionPath:   path of the current activated function
43%    .IndexRange: set the file or frame indices on which the action must be performed
44%    .FieldTransform: .TransformName: name of the selected transform function
45%                     .TransformPath:   path  of the selected transform function
46%                     .TransformHandle: corresponding function handle
47%    .InputFields: sub structure describing the input fields withfields
48%              .FieldName: name of the field
49%              .VelType: velocity type
50%              .FieldName_1: name of the second field in case of two input series
51%              .VelType_1: velocity type of the second field in case of two input series
52%    .ProjObject: %sub structure describing a projection object (read from ancillary GUI set_object)
53%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
54
55   
56function ParamOut=sub_background (Param)
57
58%% set the input elements needed on the GUI series when the action is selected in the menu ActionName
59if ~exist('Param','var') % case with no input parameter
60    ParamOut={'NbViewMax';1;...% max nbre of input file series (default='' , no limitation)
61        'AllowInputSort';'off';...% allow alphabetic sorting of the list of input files (options 'off'/'on', 'off' by default)
62        'WholeIndexRange';'on';...% prescribes the file index ranges from min to max (options 'off'/'on', 'off' by default)
63        'NbSlice';'on'; ...%nbre of slices ('off' by default)
64        'VelType';'off';...% menu for selecting the velocity type (options 'off'/'one'/'two',  'off' by default)
65        'FieldName';'off';...% menu for selecting the field (s) in the input file(options 'off'/'one'/'two', 'off' by default)
66        'FieldTransform'; 'off';...%can use a transform function
67        'ProjObject';'off';...%can use projection object(option 'off'/'on',
68        'Mask';'off';...%can use mask option   (option 'off'/'on', 'off' by default)
69        'OutputDirExt';'.sback';...%set the output dir extension
70               ''};
71        return
72end
73
74%%%%%%%%%%%% STANDARD PART (DO NOT EDIT) %%%%%%%%%%%%
75%% select different modes,  RUN, parameter input, BATCH
76% BATCH  case: read the xml file for batch case
77if ischar(Param)
78        Param=xml2struct(Param);
79        checkrun=0;
80% RUN case: parameters introduced as the input structure Param
81else
82    hseries=guidata(Param.hseries);%handles of the GUI series
83    WaitbarPos=get(hseries.waitbar_frame,'Position');%position of the waitbar on the GUI series
84    if isfield(Param,'Specific')&& strcmp(Param.Specific,'?')
85        checkrun=1;% will search input parameters (preparation of BATCH mode)
86    else
87        checkrun=2; % indicate the RUN option is used
88    end
89end
90ParamOut=Param; %default output
91
92%% root input file(s) and type
93RootPath=Param.InputTable(:,1);
94RootFile=Param.InputTable(:,3);
95SubDir=Param.InputTable(:,2);
96NomType=Param.InputTable(:,4);
97FileExt=Param.InputTable(:,5);
98
99% get the set of input file names (cell array filecell), and the lists of
100% input file or frame indices i1_series,i2_series,j1_series,j2_series
101[filecell,i1_series,i2_series,j1_series,j2_series]=get_file_series(Param);
102% filecell{iview,fileindex}: cell array representing the list of file names
103%        iview: line in the table corresponding to a given file series
104%        fileindex: file index within  the file series,
105% i1_series(iview,ref_j,ref_i)... are the corresponding arrays of indices i1,i2,j1,j2, depending on the input line iview and the two reference indices ref_i,ref_j
106% i1_series(iview,fileindex) expresses the same indices as a 1D array in file indices
107% set of frame indices used for movie or multimage input
108% numbers of slices and file indices
109
110NbSlice=1;%default
111if isfield(Param.IndexRange,'NbSlice')&&~isempty(Param.IndexRange.NbSlice)
112    NbSlice=Param.IndexRange.NbSlice;
113end
114nbview=numel(i1_series);%number of input file series (lines in InputTable)
115nbfield_j=size(i1_series{1},1); %nb of fields for the j index (bursts or volume slices)
116nbfield_i=size(i1_series{1},2); %nb of fields for the i index
117nbfield=nbfield_j*nbfield_i; %total number of fields
118nbfield_i=floor(nbfield/NbSlice);%total number of  indexes in a slice (adjusted to an integer number of slices)
119nbfield=nbfield_i*NbSlice; %total number of fields after adjustement
120
121%determine the file type on each line from the first input file
122ImageTypeOptions={'image','multimage','mmreader','video'};%allowed input file types(images)
123
124[FileType{1},FileInfo{1},MovieObject{1}]=get_file_type(filecell{1,1});
125CheckImage{1}=~isempty(find(strcmp(FileType,ImageTypeOptions)));% =1 for images
126if ~isempty(j1_series{1})
127    frame_index{1}=j1_series{1};
128else
129    frame_index{1}=i1_series{1};
130end
131
132%% calibration data and timing: read the ImaDoc files
133%not relevant here
134
135%% check coincidence in time for several input file series
136%not relevant here
137
138%% coordinate transform or other user defined transform
139%not relevant here
140
141%%%%%%%%%%%% END STANDARD PART  %%%%%%%%%%%%
142 % EDIT FROM HERE
143
144 %% check the validity of  input file types
145if CheckImage{1}
146    FileExtOut='.png'; % write result as .png images for image inputs
147    if strcmp(lower(NomType{1}(end)),'a')
148        NomTypeOut=NomType{1};%case of letter appendix
149    elseif isempty(j1_series)
150        NomTypeOut='_1';
151    else
152        NomTypeOut='_1_1';% caseof purely numerical indexing
153    end
154else
155    msgbox_uvmat('ERROR',['invalid file type input: ' FileType{1} ' not an image'])
156    return
157end
158
159%% Set field names and velocity types
160%not relevant here
161
162%% Initiate output fields
163%not relevant here
164 
165%%% SPECIFIC PART BEGINS HERE
166NbSlice=Param.IndexRange.NbSlice; %number of slices
167%siz=size(i1_series);
168nbaver_init=23;%approximate number of images used for the sliding background: to be adjusted later to include an integer number of bursts
169j1=[];%default
170
171%% adjust the proposed number of images in the sliding average to include an integer number of bursts
172if nbfield_i~=1
173    nbaver=floor(nbaver_init/nbfield_j); % number of bursts used for the sliding background,
174    if isequal(floor(nbaver/2),nbaver)
175        nbaver=nbaver+1;%put the number of burst to an odd number (so the middle burst is defined)
176    end
177    nbaver_init=nbaver*nbfield_j;%propose by default an integer number of bursts
178end
179
180%% input of specific parameters
181if checkrun %get specific parameters interactively
182    prompt = {'volume scan mode (Yes/No)';'Number of images for the sliding background (MUST FIT IN COMPUTER MEMORY)';...
183        'the luminosity rank chosen to define the background (0.1=for dense particle seeding, 0.5 (median) for sparse particles'};
184    dlg_title = ['get (slice by slice) a sliding background and substract to each image, result in subdir ' Param.OutputDir];
185    num_lines= 3;
186    def     = { 'No';num2str(nbaver_init);'0.1'};
187    answer = inputdlg(prompt,dlg_title,num_lines,def);
188   
189    %check input consistency
190    if strcmp(answer{1},'No') && ~isequal(NbSlice,1)
191        check=msgbox_uvmat('INPUT_Y-N',['confirm the multi-level splitting into ' num2str(NbSlice) ' slices']);
192        if ~strcmp(check,'Yes')
193            return
194        end
195    end
196    if strcmp(answer{1},'Yes')
197        step=1;
198    else
199        step=nbfield_j;%case of bursts: the sliding background is shifted by the length of one burst
200    end
201    nbaver_ima=str2num(answer{2});%number of images for the sliding background
202    nbaver=ceil(nbaver_ima/step);%number of bursts for the sliding background
203    if isequal(floor(nbaver/2),nbaver)
204        nbaver=nbaver+1;%set the number of bursts to an odd number (so the middle burst is defined)
205    end
206    nbaver_ima=nbaver*step;
207    if nbaver_ima > nbfield
208        msgbox_uvmat('ERROR','number of images in a slice smaller than the proposed number of images for the sliding average')
209        return
210    end
211    ParamOut.Specific.CheckVolume=strcmp(answer{1},'Yes');
212    ParamOut.Specific.SlidingSequenceSize=nbaver_ima;
213    ParamOut.Specific.BrightnessRankThreshold=str2num(answer{3});
214   
215    % apply the image rescaling function 'level' (avoid the blinking effects of bright particles)
216    answer=msgbox_uvmat('INPUT_Y-N','apply image rescaling function levels.m after sub_background');
217    ParamOut.Specific.CheckLevelTransform=strcmp(answer,'Yes');
218    if checkrun==1
219         return
220    end
221    %%%%%%%%%%%%%%%%%%%%%%  STOP HERE FOR PAMETER INPUT MODE  %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
222else
223    if isequal(Param.Specific.CheckVolume,1)
224        step=1;
225    else
226        step=nbfield_j;%case of bursts: the sliding background is shifted by the length of one burst
227    end
228    nbaver_ima=Param.Specific.SlidingSequenceSize;%number of images for the sliding background
229    nbaver=ceil(nbaver_ima/step);%number of bursts for the sliding background
230    if isequal(floor(nbaver/2),nbaver)
231        nbaver=nbaver+1;%set the number of bursts to an odd number (so the middle burst is defined)
232    end
233    nbaver_ima=nbaver*step;
234    if nbaver_ima > nbfield
235        msgbox_uvmat('ERROR','number of images in a slice smaller than the proposed number of images for the sliding average')
236        return
237    end
238end
239
240% calculate absolute brightness rank
241rank=floor(ParamOut.Specific.BrightnessRankThreshold*nbaver_ima);
242if rank==0
243    rank=1;%rank selected in the sorted image series
244end
245
246%% prealocate memory for the sliding background
247try
248    Afirst=read_image(filecell{1,1},FileType{1},MovieObject{1},frame_index{1}(1));
249    [npy,npx]=size(Afirst);
250    if strcmp(class(Afirst),'uint8') % case of 8bit images
251        Ak=zeros(npy,npx,nbaver_ima,'uint8'); %prealocate memory
252        Asort=zeros(npy,npx,nbaver_ima,'uint8'); %prealocate memory
253    else
254        Ak=zeros(npy,npx,nbaver_ima,'uint16'); %prealocate memory
255        Asort=zeros(npy,npx,nbaver_ima,'uint16'); %prealocate memory
256    end
257catch ME
258    msgbox_uvmat('ERROR',ME.message)
259    return
260end
261
262%% update the xml file
263% SubDirBase=regexprep(Param.InputTable{1,2},'\..*','');%take the root part of SubDir, before the first dot '.'
264% filexml=fullfile(RootPath{1},[SubDirBase '.xml']);
265% if ~exist(filexml,'file') && exist([fullfile(RootPath{1},SubDir{1},RootFile{1}) '.xml'],'file')% xml inside the image directory
266%     copyfile([filebase '.xml'],filexml);% copy the .xml file
267% end
268% if exist(filexml,'file')
269%     t=xmltree(filexml); 
270%     %update information on the first image name in the series
271%     uid_Heading=find(t,'ImaDoc/Heading');
272%     if isempty(uid_Heading)
273%         [t,uid_Heading]=add(t,1,'element','Heading');
274%     end
275%     uid_ImageName=find(t,'ImaDoc/Heading/ImageName');
276%     if ~isempty(j1_series{1})
277%         j1=j1_series{1}(1);
278%     end
279%     ImageName=fullfile_uvmat([dir_images term],'',RootFile{1},'.png',NomType,i1_series(1,1),[],j1);
280%     [pth,ImageName]=fileparts(ImageName);
281%     ImageName=[ImageName '.png'];
282%     if isempty(uid_ImageName)
283%         [t,uid_ImageName]=add(t,uid_Heading,'element','ImageName');
284%     end
285%     uid_value=children(t,uid_ImageName);
286%     if isempty(uid_value)
287%         t=add(t,uid_ImageName,'chardata',ImageName);%indicate  name of the first image, with ;png extension
288%     else
289%         t=set(t,uid_value(1),'value',ImageName);%indicate  name of the first image, with ;png extension
290%     end
291%     
292%     %add information about image transform
293%     [t,new_uid]=add(t,1,'element','ImageTransform');
294%     [t,NameFunction_uid]=add(t,new_uid,'element','NameFunction');
295%     [t]=add(t,NameFunction_uid,'chardata','sub_background');
296%     if GUI_config.CheckLevel
297%         [t,NameFunction_uid]=add(t,new_uid,'element','NameFunction');
298%         [t]=add(t,NameFunction_uid,'chardata','levels');
299%     end
300%     [t,NbSlice_uid]=add(t,new_uid,'element','NbSlice');
301%     [t]=add(t,new_uid,'chardata',num2str(NbSlice));
302%     [t,NbSlidingImages_uid]=add(t,new_uid,'element','NbSlidingImages');
303%     [t]=add(t,NbSlidingImages_uid,'chardata',num2str(nbaver));
304%     [t,LuminosityRank_uid]=add(t,new_uid,'element','RankBackground');
305%     [t]=add(t,LuminosityRank_uid,'chardata',num2str(rank));% luminosity rank almong the nbaver sliding images
306%     save(t,filexml)
307% end
308%copy the mask
309% if exist([filebase '_1mask_1'],'file')
310%     copyfile([filebase '_1mask_1'],[filebase_b '_1mask_1']);% copy the mask file
311% end
312
313%MAIN LOOP ON SLICES
314for islice=1:NbSlice
315    %% select the series of image indices at the level islice
316    indselect=islice:NbSlice*step:nbfield;% select file indices of the slice
317    for ifield=1:step-1
318        indselect=[indselect;indselect(end,:)+1];
319    end
320   
321    %% read the first series of nbaver_ima images and sort by luminosity at each pixel
322    for ifield = 1:nbaver_ima
323        ifile=indselect(ifield);
324        filename=filecell{1,ifile};
325        Aread=read_image(filename,FileType{1},MovieObject{1},frame_index{1}(ifile));
326        if ndims(Aread)==3;%color images
327            Aread=sum(double(Aread),3);% take the sum of color components
328        end
329        Ak(:,:,ifield)=Aread;
330    end
331    Asort=sort(Ak,3);%sort the luminosity of images at each point
332    B=Asort(:,:,rank);%background image
333    display( 'first background image will be substracted')
334    nbfirst=(ceil(nbaver/2))*step;
335    for ifield=1:nbfirst
336        Acor=double(Ak(:,:,ifield))-double(B);%substract background to the current image
337        Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
338        ifile=indselect(ifield);
339        if ~isempty(j1_series{1})
340            j1=j1_series{1}(ifile);
341        end
342        newname=fullfile_uvmat(RootPath{1},Param.OutputSubDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
343       
344        %write result file
345        if ParamOut.Specific.CheckLevelTransform
346            C=levels(Acor);
347            imwrite(C,newname,'BitDepth',8); % save the new image
348        else
349            if isequal(FileInfo{1}.BitDepth,16)
350                C=uint16(Acor);
351                imwrite(C,newname,'BitDepth',16); % save the new image
352            else
353                C=uint8(Acor);
354                imwrite(C,newname,'BitDepth',8); % save the new image
355            end
356        end
357        display([newname ' written'])
358    end
359   
360    %% repeat the operation on a sliding series of images
361    display('sliding background image will be substracted')
362    if nbfield_i > nbaver_ima
363        for ifield = step*ceil(nbaver/2)+1:step:nbfield_i-step*floor(nbaver/2)
364            if checkrun
365                stopstate=get(hseries.RUN,'BusyAction');
366                update_waitbar(hseries.waitbar_frame,WaitbarPos,(ifield+(islice-1)*nbfield_i)/(nbfield_i*NbSlice))
367            else
368                stopstate='queue';
369            end
370            if isequal(stopstate,'queue')% enable STOP command
371                Ak(:,:,1:nbaver_ima-step)=Ak(:,:,1+step:nbaver_ima);% shift the current image series by one burst (step)
372                %incorporate next burst in the current image series
373                for iburst=1:step
374                    ifile=indselect(ifield+step*floor(nbaver/2)+iburst-1);
375                    filename=fullfile_uvmat(RootPath{1},SubDir{1},RootFile{1},FileExt{1},NomType{1},i1_series{1}(ifile),[],j1_series{1}(ifile));
376                    Aread=read_image(filename,FileType{1},MovieObject{1},i1_series{1}(ifile));
377                    if ndims(Aread)==3;%color images
378                        Aread=sum(double(Aread),3);% take the sum of color components
379                    end
380                    Ak(:,:,nbaver_ima-step+iburst)=Aread;
381                end
382                Asort=sort(Ak,3);%sort the new current image series by luminosity
383                B=Asort(:,:,rank);%current background image
384                for iburst=1:step
385                    index=step*floor(nbaver/2)+iburst;
386                    Acor=double(Ak(:,:,index))-double(B);
387                    Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
388                    ifile=indselect(ifield+iburst-1);
389                    if ~isempty(j1_series{1})
390                        j1=j1_series{1}(ifile);
391                    end
392                    newname=fullfile_uvmat(RootPath{1},Param.OutputSubDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
393                    %write result file
394                    if ParamOut.Specific.CheckLevelTransform
395                        C=levels(Acor);
396                        imwrite(C,newname,'BitDepth',8); % save the new image
397                    else
398                        if isequal(FileInfo{1}.BitDepth,16)
399                            C=uint16(Acor);
400                            imwrite(C,newname,'BitDepth',16); % save the new image
401                        else
402                            C=uint8(Acor);
403                            imwrite(C,newname,'BitDepth',8); % save the new image
404                        end
405                    end
406                    display([newname ' written'])
407                   
408                end
409            else
410                return
411            end
412        end
413    end
414   
415    %% substract the background from the last images
416    display('last background image will be substracted')
417    ifield=nbfield_i-(step*ceil(nbaver/2))+1:nbfield_i;
418    for ifield=nbfield_i-(step*floor(nbaver/2))+1:nbfield_i
419        index=ifield-nbfield_i+step*(2*floor(nbaver/2)+1);
420        Acor=double(Ak(:,:,index))-double(B);
421        Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
422        ifile=indselect(ifield);
423        if ~isempty(j1_series{1})
424            j1=j1_series{1}(ifile);
425        end
426        newname=fullfile_uvmat(RootPath{1},Param.OutputSubDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
427       
428        %write result file
429        if ParamOut.Specific.CheckLevelTransform
430            C=levels(Acor);
431            imwrite(C,newname,'BitDepth',8); % save the new image
432        else
433            if isequal(FileInfo{1}.BitDepth,16)
434                C=uint16(Acor);
435                imwrite(C,newname,'BitDepth',16); % save the new image
436            else
437                C=uint8(Acor);
438                imwrite(C,newname,'BitDepth',8); % save the new image
439            end
440        end
441        display([newname ' written'])
442    end
443end
444
445%finish the waitbar
446if checkrun
447    update_waitbar(hseries.waitbar,WaitbarPos,1)
448end
449   
450
451function C=levels(A)
452%whos A;
453B=double(A(:,:,1));
454windowsize=round(min(size(B,1),size(B,2))/20);
455windowsize=floor(windowsize/2)*2+1;
456ix=1/2-windowsize/2:-1/2+windowsize/2;%
457%del=np/3;
458%fct=exp(-(ix/del).^2);
459fct2=cos(ix/(windowsize-1)/2*pi/2);
460%Mfiltre=(ones(5,5)/5^2);
461%Mfiltre=fct2';
462Mfiltre=fct2'*fct2;
463Mfiltre=Mfiltre/(sum(sum(Mfiltre)));
464
465C=filter2(Mfiltre,B);
466C(:,1:windowsize)=C(:,windowsize)*ones(1,windowsize);
467C(:,end-windowsize+1:end)=C(:,end-windowsize+1)*ones(1,windowsize);
468C(1:windowsize,:)=ones(windowsize,1)*C(windowsize,:);
469C(end-windowsize+1:end,:)=ones(windowsize,1)*C(end-windowsize,:);
470C=tanh(B./(2*C));
471[n,c]=hist(reshape(C,1,[]),100);
472% figure;plot(c,n);
473
474[m,i]=max(n);
475c_max=c(i);
476[dummy,index]=sort(abs(c-c(i)));
477n=n(index);
478c=c(index);
479i_select = find(cumsum(n)<0.95*sum(n));
480if isempty(i_select)
481    i_select = 1:length(c);
482end
483c_select=c(i_select);
484n_select=n(i_select);
485cmin=min(c_select);
486cmax=max(c_select);
487C=(C-cmin)/(cmax-cmin)*256;
488C=uint8(C);
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