source: trunk/src/series/sub_background.m @ 934

Last change on this file since 934 was 924, checked in by g7moreau, 9 years ago
  • Update Copyright Copyright 2008-2016, LEGI UMR 5519 / CNRS UGA G-INP, Grenoble, France
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1%'sub_background': substract a sliding background to an image series
2%------------------------------------------------------------------------
3% Method:
4    %calculate the background image by sorting the luminosity of each point
5    % over a sliding sub-sequence of 'nbaver_ima' images.
6    % The luminosity value of rank 'rank' is selected as the
7    % 'background'. rank=nbimages/2 gives the median value.  Smaller values are appropriate
8    % for a dense set of particles. The extrem value rank=1 gives the true minimum
9    % luminosity, but it can be polluted by noise.
10% Organization of image indices:
11    % The program is working on a series of images,
12    % In the mode 'volume', nbfield2=1 (1 image at each level)and NbSlice (=nbfield_j)
13    % Else nbfield2=nbfield_j =nbre of images in a burst (j index)
14   
15% function GUI_config=sub_background(Param)
16%
17%%%%%%%%%%% GENERAL TO ALL SERIES ACTION FCTS %%%%%%%%%%%%%%%%%%%%%%%%%%%
18%
19%OUTPUT
20% ParamOut: sets options in the GUI series.fig needed for the function
21%
22%INPUT:
23% In run mode, the input parameters are given as a Matlab structure Param copied from the GUI series.
24% In batch mode, Param is the name of the corresponding xml file containing the same information
25% when Param.Action.RUN=0 (as activated when the current Action is selected
26% in series), the function ouput paramOut set the activation of the needed GUI elements
27%
28% Param contains the elements:(use the menu bar command 'export/GUI config' in series to
29% see the current structure Param)
30%    .InputTable: cell of input file names, (several lines for multiple input)
31%                      each line decomposed as {RootPath,SubDir,Rootfile,NomType,Extension}
32%    .OutputSubDir: name of the subdirectory for data outputs
33%    .OutputDirExt: directory extension for data outputs
34%    .Action: .ActionName: name of the current activated function
35%             .ActionPath:   path of the current activated function
36%             .ActionExt: fct extension ('.m', Matlab fct, '.sh', compiled   Matlab fct
37%             .RUN =0 for GUI input, =1 for function activation
38%             .RunMode='local','background', 'cluster': type of function  use
39%             
40%    .IndexRange: set the file or frame indices on which the action must be performed
41%    .FieldTransform: .TransformName: name of the selected transform function
42%                     .TransformPath:   path  of the selected transform function
43%    .InputFields: sub structure describing the input fields withfields
44%              .FieldName: name(s) of the field
45%              .VelType: velocity type
46%              .FieldName_1: name of the second field in case of two input series
47%              .VelType_1: velocity type of the second field in case of two input series
48%              .Coord_y: name of y coordinate variable
49%              .Coord_x: name of x coordinate variable
50%    .ProjObject: %sub structure describing a projection object (read from ancillary GUI set_object)
51%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
52
53%=======================================================================
54% Copyright 2008-2016, LEGI UMR 5519 / CNRS UGA G-INP, Grenoble, France
55%   http://www.legi.grenoble-inp.fr
56%   Joel.Sommeria - Joel.Sommeria (A) legi.cnrs.fr
57%
58%     This file is part of the toolbox UVMAT.
59%
60%     UVMAT is free software; you can redistribute it and/or modify
61%     it under the terms of the GNU General Public License as published
62%     by the Free Software Foundation; either version 2 of the license,
63%     or (at your option) any later version.
64%
65%     UVMAT is distributed in the hope that it will be useful,
66%     but WITHOUT ANY WARRANTY; without even the implied warranty of
67%     MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
68%     GNU General Public License (see LICENSE.txt) for more details.
69%=======================================================================
70
71function ParamOut=sub_background (Param)
72
73%%%%%%%%%%%%%%%%%    INPUT PREPARATION MODE (no RUN)    %%%%%%%%%%%%%%%%%
74if isstruct(Param) && isequal(Param.Action.RUN,0)
75    ParamOut.AllowInputSort='off';% allow alphabetic sorting of the list of input file SubDir (options 'off'/'on', 'off' by default)
76    ParamOut.WholeIndexRange='on';% prescribes the file index ranges from min to max (options 'off'/'on', 'off' by default)
77    ParamOut.NbSlice='on'; % edit box nbre of slices made active
78    ParamOut.VelType='off';% menu for selecting the velocity type (options 'off'/'one'/'two',  'off' by default)
79    ParamOut.FieldName='off';% menu for selecting the field (s) in the input file(options 'off'/'one'/'two', 'off' by default)
80    ParamOut.FieldTransform = 'off';%can use a transform function
81    ParamOut.ProjObject='off';%can use projection object(option 'off'/'on',
82    ParamOut.Mask='off';%can use mask option   (option 'off'/'on', 'off' by default)
83    ParamOut.OutputDirExt='.sback';%set the output dir extension
84    ParamOut.OutputFileMode='NbInput';% '=NbInput': 1 output file per input file index, '=NbInput_i': 1 file per input file index i, '=NbSlice': 1 file per slice
85   
86    %% root input file(s) and type
87    % check the existence of the first file in the series
88        first_j=[];% note that the function will propose to cover the whole range of indices
89    if isfield(Param.IndexRange,'MinIndex_j'); first_j=Param.IndexRange.MinIndex_j; end
90    last_j=[];
91    if isfield(Param.IndexRange,'MaxIndex_j'); last_j=Param.IndexRange.MaxIndex_j; end
92    PairString='';
93    if isfield(Param.IndexRange,'PairString'); PairString=Param.IndexRange.PairString; end
94    [i1,i2,j1,j2] = get_file_index(Param.IndexRange.first_i,first_j,PairString);
95    FirstFileName=fullfile_uvmat(Param.InputTable{1,1},Param.InputTable{1,2},Param.InputTable{1,3},...
96        Param.InputTable{1,5},Param.InputTable{1,4},i1,i2,j1,j2);
97    if ~exist(FirstFileName,'file')
98        msgbox_uvmat('WARNING',['the first input file ' FirstFileName ' does not exist'])
99    else
100        [i1,i2,j1,j2] = get_file_index(Param.IndexRange.last_i,last_j,PairString);
101        LastFileName=fullfile_uvmat(Param.InputTable{1,1},Param.InputTable{1,2},Param.InputTable{1,3},...
102        Param.InputTable{1,5},Param.InputTable{1,4},i1,i2,j1,j2);
103        if ~exist(FirstFileName,'file')
104             msgbox_uvmat('WARNING',['the last input file ' LastFileName ' does not exist'])
105        end
106    end
107
108    %% check the validity of  input file types
109    ImageTypeOptions={'image','multimage','mmreader','video'};%allowed input file types(images)
110    FileInfo=get_file_info(FirstFileName);
111    FileType=FileInfo.FileType;
112    CheckImage=~isempty(find(strcmp(FileType,ImageTypeOptions), 1));% =1 for images
113    if ~CheckImage
114        msgbox_uvmat('ERROR',['invalid file type input: ' FileType ' not an image'])
115        return
116    end
117   
118    %% numbers of fields
119    NbSlice_i=1;%default
120    if isfield(Param.IndexRange,'NbSlice')&&~isempty(Param.IndexRange.NbSlice)
121        NbSlice_i=Param.IndexRange.NbSlice;
122    end
123    incr_j=1;%default
124    if isfield(Param.IndexRange,'incr_j')&&~isempty(Param.IndexRange.incr_j)
125        incr_j=Param.IndexRange.incr_j;
126    end
127    if isempty(first_j)||isempty(last_j)
128        nbfield_j=1;
129    else
130        nbfield_j=numel(first_j:incr_j:last_j);%nb of fields for the j index (bursts or volume slices)
131    end
132    first_i=1;last_i=1;incr_i=1;%default
133    if isfield(Param.IndexRange,'MinIndex_i'); first_i=Param.IndexRange.MinIndex_i; end   
134    if isfield(Param.IndexRange,'MaxIndex_i'); last_i=Param.IndexRange.MaxIndex_i; end
135    if isfield(Param.IndexRange,'incr_i')&&~isempty(Param.IndexRange.incr_i)
136        incr_i=Param.IndexRange.incr_i;
137    end
138    nbfield_i=numel(first_i:incr_i:last_i);%nb of fields for the i index (bursts or volume slices)
139    nbfield=nbfield_j*nbfield_i; %total number of fields
140    nbfield_i=floor(nbfield/NbSlice_i);%total number of  indexes in a slice (adjusted to an integer number of slices)
141   
142    %% setting of  parameters specific to sub_background
143    nbaver_init=23; %default number of images used for the sliding background: to be adjusted later to include an integer number of bursts 
144    if nbfield_i~=1
145        nbaver=floor(nbaver_init/nbfield_j); % number of bursts used for the sliding background,
146        if isequal(mod(nbaver,2),0)% if nbaver is even
147            nbaver=nbaver+1;%put the number of burst to an odd number (so the middle burst is defined)
148        end
149        nbaver_init=nbaver*nbfield_j;%propose by default an integer number of bursts
150    end
151   
152    prompt = {'volume scan mode (Yes/No)';'Number of images for the sliding background (MUST FIT IN COMPUTER MEMORY)';...
153        'the luminosity rank chosen to define the background (0.1=for dense particle seeding, 0.5 (median) for sparse particles'};
154    dlg_title = 'get (slice by slice) a sliding background and substract to each image';
155    num_lines= 3;
156    def     = { 'No';num2str(nbaver_init);'0.1'};
157    answer = inputdlg(prompt,dlg_title,num_lines,def);
158    if isempty(answer)
159        return
160    end
161    %check input consistency
162    if strcmp(answer{1},'No') && ~isequal(NbSlice_i,1)
163        check=msgbox_uvmat('INPUT_Y-N',['confirm the multi-level splitting into ' num2str(NbSlice_i) ' slices']);
164        if ~strcmp(check,'Yes')
165            return
166        end
167    end
168    if strcmp(answer{1},'Yes')
169        step=2;%the sliding background is shifted by the length of one burst, assumed =2 for volume ;ode
170        ParamOut.NbSlice=1; %nbre of slices displayed
171    else
172        step=nbfield_j;%case of bursts: the sliding background is shifted by the length of one burst
173    end
174    nbaver_ima=str2double(answer{2});%number of images for the sliding background
175    nbaver=ceil(nbaver_ima/step);%number of bursts for the sliding background
176    if isequal(mod(nbaver,2),0)% if nbaver is even
177        nbaver=nbaver+1;%set the number of bursts to an odd number (so the middle burst is defined)
178    end
179    nbaver_ima=nbaver*step;% correct the nbre of images corresponding to nbaver
180    ParamOut.ActionInput.CheckVolume=strcmp(answer{1},'Yes');
181    ParamOut.ActionInput.SlidingSequenceLength=nbaver_ima;
182    ParamOut.ActionInput.BrightnessRankThreshold=str2double(answer{3});
183   
184    % apply the image rescaling function 'level' (avoid the blinking effects of bright particles)
185    answer=msgbox_uvmat('INPUT_Y-N','apply image rescaling function levels.m after sub_background');
186    ParamOut.ActionInput.CheckLevelTransform=strcmp(answer,'Yes');
187    return
188end
189%%%%%%%%%%%%%%%%%    STOP HERE FOR PAMETER INPUT MODE   %%%%%%%%%%%%%%%%%
190
191%% read input parameters from an xml file if input is a file name (batch mode)
192checkrun=1;
193RUNHandle=[];
194WaitbarHandle=[];
195if ischar(Param)
196    Param=xml2struct(Param);% read Param as input file (batch case)
197    checkrun=0;
198else
199hseries=findobj(allchild(0),'Tag','series');
200RUNHandle=findobj(hseries,'Tag','RUN');%handle of RUN button in GUI series
201WaitbarHandle=findobj(hseries,'Tag','Waitbar');%handle of waitbar in GUI series
202end
203
204%% input preparation
205NbSlice_i=Param.IndexRange.NbSlice;
206if ~isequal(NbSlice_i,1)
207    display(['multi-level splitting into ' num2str(NbSlice_i) ' slices']);
208end
209RootPath=Param.InputTable(:,1);
210RootFile=Param.InputTable(:,3);
211SubDir=Param.InputTable(:,2);
212NomType=Param.InputTable(:,4);
213FileExt=Param.InputTable(:,5);
214%hdisp=disp_uvmat('WAITING...','checking the file series',checkrun);
215[filecell,i1_series,i2_series,j1_series]=get_file_series(Param);
216% if ~isempty(hdisp),delete(hdisp),end;
217%%%%%%%%%%%%
218    % The cell array filecell is the list of input file names, while
219    % filecell{iview,fileindex}:
220    %        iview: line in the table corresponding to a given file series
221    %        fileindex: file index within  the file series,
222    % i1_series(iview,ref_j,ref_i)... are the corresponding arrays of indices i1,i2,j1,j2, depending on the input line iview and the two reference indices ref_i,ref_j
223    % i1_series(iview,fileindex) expresses the same indices as a 1D array in file indices
224%%%%%%%%%%%%
225[FileInfo{1},MovieObject{1}]=get_file_info(filecell{1,1});
226FileType{1}=FileInfo{1}.FileType;
227    if ~isempty(j1_series{1})
228        frame_index{1}=j1_series{1};
229    else
230        frame_index{1}=i1_series{1};
231    end
232
233
234%% output file naming
235FileExtOut='.png'; % write result as .png images for image inputs
236if strcmp(lower(NomType{1}(end)),'a')
237    NomTypeOut=NomType{1};%case of letter appendix
238elseif isempty(j1_series{1})
239    NomTypeOut='_1';
240else
241    NomTypeOut='_1_1';% caseof purely numerical indexing
242end
243OutputDir=[Param.OutputSubDir Param.OutputDirExt];
244
245%% file index parameters
246% NbSlice_i: nbre of slices for i index: different of of 1 for multi-level,
247% the function sub_background is then relaunched by the GUI series for each
248%      slice, incrementing the first index i by 1
249% NbSlice_j: nbre of slices in volume mode
250% nbfield : total number of images treated per slice
251% step: shift of image index at each step of the sliding background (corresponding to the nbre of images in a burst)
252% nbaver_ima: nbre of the images in the sliding sequence used for the background
253% nbaver=nbaver_ima/step: nbre of bursts corresponding to nbaver_ima images. It has been adjusted so that nbaver is an odd integer
254nbfield_j=size(i1_series{1},1); %nb of fields for the j index (bursts or volume slices)
255nbfield_i=size(i1_series{1},2); %nb of fields for the i index
256
257if Param.ActionInput.CheckVolume
258    step=2;% we assume the burst contains only one image pair
259    NbSlice_j=nbfield_j;
260    NbSlice=nbfield_j;
261    nbfield_series=nbfield_i;
262else
263    step=nbfield_j;%case of bursts: the sliding background is shifted by the length of one burst
264        NbSlice_j=1;
265        NbSlice=NbSlice_i;
266    %nbfield_i=floor(nbfield_i/NbSlice_i);%total number of  indexes in a slice (adjusted to an integer number of slices)
267    %nbfield=nbfield_i*NbSlice_i; %total number of fields after adjustement
268    nbfield_series=nbfield_i*nbfield_j;
269end
270nbfield=nbfield_j*nbfield_i; %total number of fields
271nbaver_ima=Param.ActionInput.SlidingSequenceLength;%number of images for the sliding background
272nbaver=ceil(nbaver_ima/step);%number of bursts for the sliding background
273if isequal(mod(nbaver,2),0)
274    nbaver=nbaver+1;%set the number of bursts to an odd number (so the middle burst is defined)
275end
276nbaver_ima=nbaver*step;
277if nbaver_ima > nbfield
278    display('number of images in a slice smaller than the proposed number of images for the sliding average')
279    return
280end
281halfnbaver=floor(nbaver/2); % half width (in unit of bursts) of the sliding background
282
283%% calculate absolute brightness rank
284rank=floor(Param.ActionInput.BrightnessRankThreshold*nbaver_ima);
285if rank==0
286    rank=1;%rank selected in the sorted image series
287end
288
289%% prealocate memory for the sliding background
290try
291    Afirst=read_image(filecell{1,1},FileType{1},MovieObject{1},frame_index{1}(1));
292    [npy,npx,nbcolor]=size(Afirst);% the argument nbcolor is important to get npx right for color images
293    if strcmp(class(Afirst),'uint8') % case of 8bit images
294        Ak=zeros(npy,npx,nbaver_ima,'uint8'); %prealocate memory
295        Asort=zeros(npy,npx,nbaver_ima,'uint8'); %prealocate memory
296    else
297        Ak=zeros(npy,npx,nbaver_ima,'uint16'); %prealocate memory
298        Asort=zeros(npy,npx,nbaver_ima,'uint16'); %prealocate memory
299    end
300catch ME
301    msgbox_uvmat('ERROR',['sub_background/read_image/' ME.message])
302    return
303end
304
305
306%%%%%%%  LOOP ON SLICES FOR VOLUME SCAN %%%%%%%
307for j_slice=1:NbSlice_j
308    %% select the series of i indices to process
309    indselect=j_slice:step*NbSlice_j:nbfield;% select file indices of the slice
310    for ifield=1:step-1
311        indselect=[indselect;indselect(end,:)+NbSlice_j];
312    end
313   
314    %% read the first series of nbaver_ima images and sort by luminosity at each pixel
315    for ifield = 1:nbaver_ima
316        ifile=indselect(ifield);
317        filename=filecell{1,ifile};
318        Aread=read_image(filename,FileType{1},MovieObject{1},frame_index{1}(ifile));
319        if ndims(Aread)==3;%color images
320            Aread=sum(double(Aread),3);% take the sum of color components
321        end
322        Ak(:,:,ifield)=Aread;
323    end
324    Asort=sort(Ak,3);%sort the luminosity of images at each point
325    B=Asort(:,:,rank);%background image
326   
327    %% substract the first background image to the first images
328    display( 'first background image will be substracted')
329    for ifield=1:step*(halfnbaver+1);% nbre of images treated by the first background image
330        Acor=double(Ak(:,:,ifield))-double(B);%substract background to the current image
331        Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
332        ifile=indselect(ifield);
333        j1=1;
334        if ~isempty(j1_series{1})
335            j1=j1_series{1}(ifile);
336        end
337        newname=fullfile_uvmat(RootPath{1},OutputDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
338       
339        %write result file
340        if Param.ActionInput.CheckLevelTransform
341            C=levels(Acor);
342            imwrite(C,newname,'BitDepth',8); % save the new image
343        else
344            if isequal(FileInfo{1}.BitDepth,16)
345                C=uint16(Acor);
346                imwrite(C,newname,'BitDepth',16); % save the new image
347            else
348                C=uint8(Acor);
349                imwrite(C,newname,'BitDepth',8); % save the new image
350            end
351        end
352        display([newname ' written'])
353    end
354   
355    %% repeat the operation on a sliding series of images
356    display('sliding background image will be substracted')
357    if nbfield_series > nbaver_ima
358        for ifield = step*(halfnbaver+1):step:nbfield_series-step*(halfnbaver+1)% ifield +iburst=index of the current processed image
359            update_waitbar(WaitbarHandle,ifield/nbfield_series)
360            if  ~isempty(RUNHandle)&&~strcmp(get(RUNHandle,'BusyAction'),'queue')
361                disp('program stopped by user')
362                return
363            end
364            if nbaver_ima>step
365            Ak(:,:,1:nbaver_ima-step)=Ak(:,:,1+step:nbaver_ima);% shift the current image series by one burst (step)
366            end
367            %incorporate next burst in the current image series
368            for iburst=1:step
369                ifile=indselect(ifield+iburst+step*halfnbaver);
370                j1=1;
371                if ~isempty(j1_series{1})
372                    j1=j1_series{1}(ifile);
373                end
374                filename=fullfile_uvmat(RootPath{1},SubDir{1},RootFile{1},FileExt{1},NomType{1},i1_series{1}(ifile),[],j1);
375                Aread=read_image(filename,FileType{1},MovieObject{1},i1_series{1}(ifile));
376                if ndims(Aread)==3;%color images
377                    Aread=sum(double(Aread),3);% take the sum of color components
378                end
379                Ak(:,:,nbaver_ima-step+iburst)=Aread;% fill the last burst of the current image series by the new image
380            end
381            Asort=sort(Ak,3);%sort the new current image series by luminosity
382            B=Asort(:,:,rank);%current background image
383            %substract the background for the current burst
384            for iburst=1:step
385                Acor=double(Ak(:,:,step*halfnbaver+iburst))-double(B); %the current image has been already read ans stored as index step*halfnbaver+iburst in the current series
386                Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
387                ifile=indselect(ifield+iburst);
388                if ~isempty(j1_series{1})
389                    j1=j1_series{1}(ifile);
390                end
391                newname=fullfile_uvmat(RootPath{1},OutputDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
392                %write result file
393                if Param.ActionInput.CheckLevelTransform
394                    C=levels(Acor);
395                    imwrite(C,newname,'BitDepth',8); % save the new image
396                else
397                    if isequal(FileInfo{1}.BitDepth,16)
398                        C=uint16(Acor);
399                        imwrite(C,newname,'BitDepth',16); % save the new image
400                    else
401                        C=uint8(Acor);
402                        imwrite(C,newname,'BitDepth',8); % save the new image
403                    end
404                end
405                display([newname ' written'])
406            end
407        end
408    end
409   
410    %% substract the background from the last images
411    display('last background image will be substracted')
412    for  ifield=nbfield_series-step*halfnbaver+1:nbfield_series
413        Acor=double(Ak(:,:,ifield-nbfield_series+step*(2*halfnbaver+1)))-double(B);
414        Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
415        ifile=indselect(ifield);
416        if ~isempty(j1_series{1})
417            j1=j1_series{1}(ifile);
418        end
419        newname=fullfile_uvmat(RootPath{1},OutputDir,RootFile{1},FileExtOut,NomTypeOut,i1_series{1}(ifile),[],j1);
420        %write result file
421        if Param.ActionInput.CheckLevelTransform
422            C=levels(Acor);
423            imwrite(C,newname,'BitDepth',8); % save the new image
424        else
425            if isequal(FileInfo{1}.BitDepth,16)
426                C=uint16(Acor);
427                imwrite(C,newname,'BitDepth',16); % save the new image
428            else
429                C=uint8(Acor);
430                imwrite(C,newname,'BitDepth',8); % save the new image
431            end
432        end
433        display([newname ' written'])
434    end
435end
436
437
438function C=levels(A)
439%whos A;
440B=double(A(:,:,1));
441windowsize=round(min(size(B,1),size(B,2))/20);
442windowsize=floor(windowsize/2)*2+1;
443ix=1/2-windowsize/2:-1/2+windowsize/2;%
444%del=np/3;
445%fct=exp(-(ix/del).^2);
446fct2=cos(ix/(windowsize-1)/2*pi/2);
447%Mfiltre=(ones(5,5)/5^2);
448%Mfiltre=fct2';
449Mfiltre=fct2'*fct2;
450Mfiltre=Mfiltre/(sum(sum(Mfiltre)));
451
452C=filter2(Mfiltre,B);
453C(:,1:windowsize)=C(:,windowsize)*ones(1,windowsize);
454C(:,end-windowsize+1:end)=C(:,end-windowsize+1)*ones(1,windowsize);
455C(1:windowsize,:)=ones(windowsize,1)*C(windowsize,:);
456C(end-windowsize+1:end,:)=ones(windowsize,1)*C(end-windowsize,:);
457C=tanh(B./(2*C));
458[n,c]=hist(reshape(C,1,[]),100);
459% figure;plot(c,n);
460
461[m,i]=max(n);
462c_max=c(i);
463[dummy,index]=sort(abs(c-c(i)));
464n=n(index);
465c=c(index);
466i_select = find(cumsum(n)<0.95*sum(n));
467if isempty(i_select)
468    i_select = 1:length(c);
469end
470c_select=c(i_select);
471n_select=n(i_select);
472cmin=min(c_select);
473cmax=max(c_select);
474C=(C-cmin)/(cmax-cmin)*256;
475C=uint8(C);
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