[950] | 1 | % 'ima_remove_background': removes backgound from an image (using the local minimum)
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| 2 | % requires the Matlab image processing toolbox
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| 3 | %------------------------------------------------------------------------
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| 4 | %%%% Use the general syntax for transform fields with a single input %%%%
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| 5 | % OUTPUT:
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| 6 | % DataOut: output field structure
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| 7 | %
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| 8 | %INPUT:
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| 9 | % DataIn: first input field structure
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| 10 |
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[810] | 11 | %=======================================================================
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[1107] | 12 | % Copyright 2008-2022, LEGI UMR 5519 / CNRS UGA G-INP, Grenoble, France
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[810] | 13 | % http://www.legi.grenoble-inp.fr
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| 14 | % Joel.Sommeria - Joel.Sommeria (A) legi.cnrs.fr
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| 15 | %
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| 16 | % This file is part of the toolbox UVMAT.
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| 17 | %
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| 18 | % UVMAT is free software; you can redistribute it and/or modify
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| 19 | % it under the terms of the GNU General Public License as published
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| 20 | % by the Free Software Foundation; either version 2 of the license,
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| 21 | % or (at your option) any later version.
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| 22 | %
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| 23 | % UVMAT is distributed in the hope that it will be useful,
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| 24 | % but WITHOUT ANY WARRANTY; without even the implied warranty of
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| 25 | % MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
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| 26 | % GNU General Public License (see LICENSE.txt) for more details.
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| 27 | %=======================================================================
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| 28 |
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[950] | 29 | function DataOut=ima_remove_background_blocks(DataIn,Param)
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| 30 | %------------------------------------------------------------------------
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| 31 | %% request input parameters
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| 32 | if isfield(DataIn,'Action') && isfield(DataIn.Action,'RUN') && isequal(DataIn.Action.RUN,0)
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| 33 | prompt = {'block size(pixels)'};
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| 34 | dlg_title = 'get the block size (in pixels) used to calculate the local statistics';
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| 35 | num_lines= 1;
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| 36 | def = { '100'};
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| 37 | if isfield(Param,'TransformInput')&&isfield(Param.TransformInput,'BlockSize')
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| 38 | def={num2str(Param.TransformInput.BlockSize)};
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| 39 | end
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| 40 | answer = inputdlg(prompt,dlg_title,num_lines,def);
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| 41 | DataOut.TransformInput.BlockSize=str2num(answer{1});
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[574] | 42 | return
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| 43 | end
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[950] | 44 | if ~isfield(DataIn,'A')
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| 45 | DataOut.Txt='remove_particles only valid for input images';
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| 46 | return
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| 47 | end
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| 48 | if ~exist('imerode','file');
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| 49 | DataOut.Txt='the function imerode from the image processing toolbox is needed';
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| 50 | return
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| 51 | end
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| 52 |
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[574] | 53 | %---------------------------------------------------------
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| 54 | DataOut=DataIn;%default
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[950] | 55 | nblock_y=2*Param.TransformInput.BlockSize;
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| 56 | nblock_x=2*Param.TransformInput.BlockSize;
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| 57 | [npy,npx]=size(DataIn.A);
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| 58 | [X,Y]=meshgrid(1:npx,1:npy);
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[574] | 59 |
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[950] | 60 | %BACKGROUND LEVEL
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| 61 | Atype=class(DataIn.A);
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| 62 | A=double(DataIn.A);
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| 63 | %Backg=zeros(size(A));
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| 64 | %Aflagmin=sparse(imregionalmin(A));%Amin=1 for local image minima
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| 65 | %Amin=A.*Aflagmin;%values of A at local minima
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| 66 | % local background: find all the local minima in image subblocks
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| 67 | fctblock= inline('median(x(:))');
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| 68 | Backg=blkproc(A,[nblock_y nblock_x],fctblock);% take the median in blocks
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| 69 | fctblock= inline('mean(x(:))');
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| 70 | B=imresize(Backg,size(A),'bilinear');% interpolate to the initial size image
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[1097] | 71 | DataOut.A=B;
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| 72 |
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| 73 | % A=(A-B);%substract background
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| 74 | % AMean=blkproc(A,[nblock_y nblock_x],fctblock);% take the mean in blocks
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| 75 | % fctblock= inline('var(x(:))');
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| 76 | % AVar=blkproc(A,[nblock_y nblock_x],fctblock);% take the mean in blocks
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| 77 | % Avalue=AVar./AMean;% typical value of particle luminosity
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| 78 | % Avalue=imresize(Avalue,size(A),'bilinear');% interpolate to the initial size image
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| 79 | % DataOut.A=uint16(1000*tanh(A./(2*Avalue)));
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[950] | 80 | %Bmin=blkproc(Aflagmin,[nblock_y nblock_x],sumblock);% find the number of minima in blocks
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| 81 | %Backg=Backg./Bmin; % find the average of minima in blocks
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[574] | 82 |
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| 83 |
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[1097] | 84 |
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| 85 |
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