source: trunk/src/series/sub_background.m @ 1183

Last change on this file since 1183 was 1180, checked in by sommeria, 9 months ago

various bugs repaired,Relabeling frames installed for multitif

File size: 22.8 KB
RevLine 
[457]1%'sub_background': substract a sliding background to an image series
[169]2%------------------------------------------------------------------------
[24]3% Method:
4    %calculate the background image by sorting the luminosity of each point
5    % over a sliding sub-sequence of 'nbaver_ima' images.
6    % The luminosity value of rank 'rank' is selected as the
7    % 'background'. rank=nbimages/2 gives the median value.  Smaller values are appropriate
8    % for a dense set of particles. The extrem value rank=1 gives the true minimum
9    % luminosity, but it can be polluted by noise.
10% Organization of image indices:
[454]11    % The program is working on a series of images,
[1178]12    % In the mode 'volume', nbfield2=1 (1 image at each level)and NbSlice (=NbField_j)
13    % Else nbfield2=NbField_j =nbre of images in a burst (j index)
[451]14   
[457]15% function GUI_config=sub_background(Param)
[169]16%
[451]17%%%%%%%%%%% GENERAL TO ALL SERIES ACTION FCTS %%%%%%%%%%%%%%%%%%%%%%%%%%%
[457]18%
[451]19%OUTPUT
[596]20% ParamOut: sets options in the GUI series.fig needed for the function
[451]21%
22%INPUT:
23% In run mode, the input parameters are given as a Matlab structure Param copied from the GUI series.
24% In batch mode, Param is the name of the corresponding xml file containing the same information
[596]25% when Param.Action.RUN=0 (as activated when the current Action is selected
26% in series), the function ouput paramOut set the activation of the needed GUI elements
[451]27%
[596]28% Param contains the elements:(use the menu bar command 'export/GUI config' in series to
29% see the current structure Param)
[451]30%    .InputTable: cell of input file names, (several lines for multiple input)
31%                      each line decomposed as {RootPath,SubDir,Rootfile,NomType,Extension}
32%    .OutputSubDir: name of the subdirectory for data outputs
[596]33%    .OutputDirExt: directory extension for data outputs
[451]34%    .Action: .ActionName: name of the current activated function
35%             .ActionPath:   path of the current activated function
[596]36%             .ActionExt: fct extension ('.m', Matlab fct, '.sh', compiled   Matlab fct
37%             .RUN =0 for GUI input, =1 for function activation
38%             .RunMode='local','background', 'cluster': type of function  use
39%             
[451]40%    .IndexRange: set the file or frame indices on which the action must be performed
41%    .FieldTransform: .TransformName: name of the selected transform function
42%                     .TransformPath:   path  of the selected transform function
43%    .InputFields: sub structure describing the input fields withfields
[596]44%              .FieldName: name(s) of the field
[451]45%              .VelType: velocity type
46%              .FieldName_1: name of the second field in case of two input series
47%              .VelType_1: velocity type of the second field in case of two input series
[596]48%              .Coord_y: name of y coordinate variable
49%              .Coord_x: name of x coordinate variable
[451]50%    .ProjObject: %sub structure describing a projection object (read from ancillary GUI set_object)
51%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
[810]52
53%=======================================================================
[1126]54% Copyright 2008-2024, LEGI UMR 5519 / CNRS UGA G-INP, Grenoble, France
[810]55%   http://www.legi.grenoble-inp.fr
[1127]56%   Joel.Sommeria - Joel.Sommeria (A) univ-grenoble-alpes.fr
[810]57%
58%     This file is part of the toolbox UVMAT.
59%
60%     UVMAT is free software; you can redistribute it and/or modify
61%     it under the terms of the GNU General Public License as published
62%     by the Free Software Foundation; either version 2 of the license,
63%     or (at your option) any later version.
64%
65%     UVMAT is distributed in the hope that it will be useful,
66%     but WITHOUT ANY WARRANTY; without even the implied warranty of
67%     MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
68%     GNU General Public License (see LICENSE.txt) for more details.
69%=======================================================================
70
[459]71function ParamOut=sub_background (Param)
[24]72
[676]73%%%%%%%%%%%%%%%%%    INPUT PREPARATION MODE (no RUN)    %%%%%%%%%%%%%%%%%
[592]74if isstruct(Param) && isequal(Param.Action.RUN,0)
[605]75    ParamOut.AllowInputSort='off';% allow alphabetic sorting of the list of input file SubDir (options 'off'/'on', 'off' by default)
76    ParamOut.WholeIndexRange='on';% prescribes the file index ranges from min to max (options 'off'/'on', 'off' by default)
[897]77    ParamOut.NbSlice='on'; % edit box nbre of slices made active
[605]78    ParamOut.VelType='off';% menu for selecting the velocity type (options 'off'/'one'/'two',  'off' by default)
79    ParamOut.FieldName='off';% menu for selecting the field (s) in the input file(options 'off'/'one'/'two', 'off' by default)
[1180]80    ParamOut.FieldTransform = 'off';%can use a transform function (option 'off'/'on','off' by default)
81    ParamOut.ProjObject='off';%cannot use projection object(option 'off'/'on','off' by default)
82    ParamOut.Mask='off';%cannot use mask option   (option 'off'/'on', 'off' by default)
[592]83    ParamOut.OutputDirExt='.sback';%set the output dir extension
[605]84    ParamOut.OutputFileMode='NbInput';% '=NbInput': 1 output file per input file index, '=NbInput_i': 1 file per input file index i, '=NbSlice': 1 file per slice
[716]85
[592]86    %% check the validity of  input file types
[1178]87    if isfield(Param,'SeriesData')&& isfield(Param.SeriesData,'FileInfo')
88    if ~strcmp(Param.SeriesData.FileInfo{1}.FieldType,'image')
89        msgbox_uvmat('ERROR','invalid file type input: not an image series')
[451]90        return
[592]91    end
[1178]92    end
93
[676]94    %% numbers of fields
[897]95    NbSlice_i=1;%default
[676]96    if isfield(Param.IndexRange,'NbSlice')&&~isempty(Param.IndexRange.NbSlice)
[897]97        NbSlice_i=Param.IndexRange.NbSlice;
[592]98    end
[774]99    incr_j=1;%default
100    if isfield(Param.IndexRange,'incr_j')&&~isempty(Param.IndexRange.incr_j)
101        incr_j=Param.IndexRange.incr_j;
102    end
[1178]103    if isfield(Param.IndexRange,'first_j')&&~isempty(Param.IndexRange.first_j)
104        NbField_j=numel(Param.IndexRange.first_j:incr_j:Param.IndexRange.last_j);%nb of fields for the j index (bursts or volume slices)
[774]105    else
[1178]106        NbField_j=1;
[774]107    end
[897]108    first_i=1;last_i=1;incr_i=1;%default
[917]109    if isfield(Param.IndexRange,'MinIndex_i'); first_i=Param.IndexRange.MinIndex_i; end   
110    if isfield(Param.IndexRange,'MaxIndex_i'); last_i=Param.IndexRange.MaxIndex_i; end
[774]111    if isfield(Param.IndexRange,'incr_i')&&~isempty(Param.IndexRange.incr_i)
112        incr_i=Param.IndexRange.incr_i;
113    end
114    nbfield_i=numel(first_i:incr_i:last_i);%nb of fields for the i index (bursts or volume slices)
[1178]115    nbfield=NbField_j*nbfield_i; %total number of fields
[897]116    nbfield_i=floor(nbfield/NbSlice_i);%total number of  indexes in a slice (adjusted to an integer number of slices)
[592]117   
[676]118    %% setting of  parameters specific to sub_background
[1058]119    CheckVolume='No';
120    nbaver_init=23; %default number of images used for the sliding background: to be adjusted later to include an integer number of bursts
[1124]121    SaturationValue=0;
[1178]122     if nbfield_i~=1 && NbField_j<=nbaver_init
123        nbaver=floor(nbaver_init/NbField_j); % number of bursts used for the sliding background,
[854]124        if isequal(mod(nbaver,2),0)% if nbaver is even
[592]125            nbaver=nbaver+1;%put the number of burst to an odd number (so the middle burst is defined)
126        end
[1178]127        nbaver_init=nbaver*NbField_j;%propose by default an integer number of bursts
[592]128    end
[1058]129    BrightnessRankThreshold=0.1;
130    if isfield(Param,'ActionInput')
131        if isfield(Param.ActionInput,'CheckVolume') && Param.ActionInput.CheckVolume
132            CheckVolume='Yes';
133        end
134        if isfield(Param.ActionInput,'SlidingSequenceLength')
135         nbaver_init=Param.ActionInput.SlidingSequenceLength;
136        end
137        if isfield(Param.ActionInput,'BrightnessRankThreshold')
138          BrightnessRankThreshold=Param.ActionInput.BrightnessRankThreshold;
139        end
[1124]140        if isfield(Param.ActionInput,'SaturationValue')
141            SaturationValue=Param.ActionInput.SaturationValue;
[1058]142        end
143    end   
[951]144    prompt = {'volume scan mode (Yes/No)';...
145        'Number of images for the sliding background (MUST FIT IN COMPUTER MEMORY)';...
146        'the luminosity rank chosen to define the background (0.1=for dense particle seeding, 0.5 (median) for sparse particles';...
[1124]147        'image saturation level for rescaling( reduce the influence of particles brighter than this value), =0 for no rescaling' };
[676]148    dlg_title = 'get (slice by slice) a sliding background and substract to each image';
[1124]149    num_lines= 4;
150    def     = { CheckVolume;num2str(nbaver_init);num2str(BrightnessRankThreshold);num2str(SaturationValue)};
[676]151    answer = inputdlg(prompt,dlg_title,num_lines,def);
[917]152    if isempty(answer)
153        return
154    end
[676]155    %check input consistency
[897]156    if strcmp(answer{1},'No') && ~isequal(NbSlice_i,1)
157        check=msgbox_uvmat('INPUT_Y-N',['confirm the multi-level splitting into ' num2str(NbSlice_i) ' slices']);
[676]158        if ~strcmp(check,'Yes')
159            return
[592]160        end
161    end
[676]162    if strcmp(answer{1},'Yes')
[1124]163        step=2;%the sliding background is shifted by the length of one burst, assumed =2 for volume
[897]164        ParamOut.NbSlice=1; %nbre of slices displayed
[676]165    else
[1178]166        step=NbField_j;%case of bursts: the sliding background is shifted by the length of one burst
[676]167    end
[1178]168    ParamOut.ActionInput.SlidingSequenceLength=adjust_slidinglength(str2double(answer{2}),step);
[599]169    ParamOut.ActionInput.CheckVolume=strcmp(answer{1},'Yes');
[854]170    ParamOut.ActionInput.BrightnessRankThreshold=str2double(answer{3});
[1124]171%     ParamOut.ActionInput.CheckSubmedian=strcmp(answer{4},'Yes');
172    ParamOut.ActionInput.SaturationValue=str2double(answer{4});
[592]173    % apply the image rescaling function 'level' (avoid the blinking effects of bright particles)
[951]174%     answer=msgbox_uvmat('INPUT_Y-N','apply image rescaling function levels.m after sub_background');
175%     ParamOut.ActionInput.CheckLevelTransform=strcmp(answer,'Yes');
[592]176    return
[24]177end
[676]178%%%%%%%%%%%%%%%%%    STOP HERE FOR PAMETER INPUT MODE   %%%%%%%%%%%%%%%%%
[24]179
[592]180%% read input parameters from an xml file if input is a file name (batch mode)
[1178]181% checkrun=1;
[904]182RUNHandle=[];
[1178]183% WaitbarHandle=[];
[457]184if ischar(Param)
[592]185    Param=xml2struct(Param);% read Param as input file (batch case)
[904]186else
[1178]187 hseries=findobj(allchild(0),'Tag','series');
[624]188RUNHandle=findobj(hseries,'Tag','RUN');%handle of RUN button in GUI series
[1178]189% WaitbarHandle=findobj(hseries,'Tag','Waitbar');%handle of waitbar in GUI series
[904]190end
[676]191
192%% input preparation
[897]193NbSlice_i=Param.IndexRange.NbSlice;
194if ~isequal(NbSlice_i,1)
[1178]195    disp(['multi-level splitting into ' num2str(NbSlice_i) ' slices']);
[592]196end
[1178]197RootPath=Param.InputTable{1,1};
198RootFile=Param.InputTable{1,3};
199SubDir=Param.InputTable{1,2};
200NomType=Param.InputTable{1,4};
201FileExt=Param.InputTable{1,5};
202%[filecell,i1_series,i2_series,j1_series]=get_file_series(Param);%series of file names organised as a single array
[1172]203
[451]204
[897]205%% file index parameters
[1124]206% NbSlice_i: nbre of slices for i index in multi-level mode: equal to 1 for a single level
[897]207% the function sub_background is then relaunched by the GUI series for each
208%      slice, incrementing the first index i by 1
209% NbSlice_j: nbre of slices in volume mode
210% nbfield : total number of images treated per slice
211% step: shift of image index at each step of the sliding background (corresponding to the nbre of images in a burst)
212% nbaver_ima: nbre of the images in the sliding sequence used for the background
213% nbaver=nbaver_ima/step: nbre of bursts corresponding to nbaver_ima images. It has been adjusted so that nbaver is an odd integer
[1178]214i_indices=Param.IndexRange.first_i:Param.IndexRange.incr_i:Param.IndexRange.last_i;
215if isfield(Param.IndexRange,'first_j')
216j_indices=Param.IndexRange.first_j:Param.IndexRange.incr_j:Param.IndexRange.last_j;
217else
218    j_indices=1;
219end
220nbfield_i=numel(i_indices); %nb of fields for the i index (bursts or volume slices)
221NbField_j=numel(j_indices); %nb of fields for the j index
222j_indices=j_indices'*ones(1,nbfield_i);
223i_indices=ones(NbField_j,1)*i_indices;
[917]224
[1124]225if Param.ActionInput.CheckVolume% case of volume scan: the background images must be determined for each index j
[897]226    step=2;% we assume the burst contains only one image pair
[1178]227    NbSlice_j=NbField_j;
[897]228    nbfield_series=nbfield_i;
229else
[1178]230    if Param.ActionInput.SlidingSequenceLength<NbField_j
231        step=1;
232    else
233    step=NbField_j;%case of bursts: the sliding background is shifted by the length of one burst
234    end
[1124]235    NbSlice_j=1;
[1178]236    nbfield_series=nbfield_i*NbField_j;
[54]237end
[1178]238nbfield=NbField_j*nbfield_i; %total number of fields
[1124]239[nbaver_ima,nbaver,step]=adjust_slidinglength(Param.ActionInput.SlidingSequenceLength,step);
[592]240if nbaver_ima > nbfield
[1178]241    disp('number of images in a slice smaller than the proposed number of images for the sliding average')
[592]242    return
[24]243end
[897]244halfnbaver=floor(nbaver/2); % half width (in unit of bursts) of the sliding background
[454]245
[1178]246
247%% File relabeling documented by the xml file
248CheckRelabel=isfield(Param,'FileSeries' );
249
250%% Input file info
251if CheckRelabel
[1180]252    [RootFileOut,frame_index]=index2filename(Param.FileSeries,Param.IndexRange.first_i,j_indices(1),NbField_j);
253    FirstFileName=fullfile(RootPath,SubDir,RootFileOut);
[1178]254else
[1180]255    FirstFileName=fullfile_uvmat(RootPath,SubDir,RootFile,FileExt,NomType,Param.IndexRange.first_i,[],j_indices(1));%get first file name
256    RootFileOut=RootFile;
[1178]257end
258[FileInfo,MovieObject]=get_file_info(FirstFileName);
259FileType=FileInfo.FileType;
[1180]260if ~CheckRelabel
261    if isfield(FileInfo,'NumberOfFrames') && FileInfo.NumberOfFrames >1
262        if isempty(regexp(NomType,'1$', 'once'))% no file indexing
263            frame_index=i_indices;% the index i denotes the frame number in a movie, no index j
264        else
265            frame_index=j_indices;% the index j denotes the frame number in a movie
266            MovieObject=[]; %not a single video object
267        end
[1178]268    else
[1180]269        frame_index=ones(1,nbfield);
[1178]270    end
271end
272
273%% output file naming
274FileExtOut='.png'; % write result as .png images for image inputsFileInfo.FileType='image'
275if strcmp(FileInfo.FileType,'image')
276    NomTypeOut=NomType;
277elseif NbField_j==1
278    NomTypeOut='_1';
279else
280    NomTypeOut='_1_1';% case of purely numerical indexing
281end
282OutputDir=[Param.OutputSubDir Param.OutputDirExt];
283OutputPath=fullfile(Param.OutputPath,Param.Experiment,Param.Device);
284
[897]285%% calculate absolute brightness rank
[599]286rank=floor(Param.ActionInput.BrightnessRankThreshold*nbaver_ima);
[24]287if rank==0
288    rank=1;%rank selected in the sorted image series
289end
290
[239]291%% prealocate memory for the sliding background
[1180]292Ak=zeros(FileInfo.Height,FileInfo.Width,nbaver_ima,['uint' num2str(FileInfo.BitDepth)]); %prealocate memory
[239]293
[1178]294%% selection of frame indices
[1180]295if Param.ActionInput.CheckVolume
[1172]296    nbfield=floor(nbfield/NbSlice_j)*NbSlice_j;% truncate the total number of frames in case of incomplete series
297    indselect=1:nbfield;
[1180]298    indselect=reshape(indselect,NbSlice_j,[]);
299    NbSlice=NbSlice_j;
[1172]300else
[1180]301    NbSlice=NbSlice_i;
[1172]302    nbfield=floor(nbfield/NbSlice)*NbSlice;% truncate the total number of frames in case of incomplete series
303    indselect=reshape(1:nbfield,NbSlice,[]);
304    for j_slice=1:NbSlice
[1180]305        indselect(j_slice,:)=j_slice:NbSlice:nbfield;% select file indices of the slice
[1172]306    end
307end
[24]308
[1172]309%%%%%%%  LOOP ON SLICES %%%%%%%
310for j_slice=1:NbSlice
[1178]311
[897]312    %% read the first series of nbaver_ima images and sort by luminosity at each pixel
313    for ifield = 1:nbaver_ima
[1178]314        ifile=indselect(j_slice,ifield);
315        %filename=filecell{1,ifile};
316        if CheckRelabel
[1180]317            [filename,FrameIndex]=index2filename(Param.FileSeries,i_indices(ifile),j_indices(ifile),NbField_j);
318             filename=fullfile(RootPath,SubDir,filename);
[1178]319        else
320            filename=fullfile_uvmat(RootPath,SubDir,RootFile,FileExt,NomType,i_indices(ifile),[],j_indices(ifile));
321            FrameIndex=frame_index(ifile);
322        end
323        Aread=read_image(filename,FileType,MovieObject,FrameIndex);
[1124]324        if ndims(Aread)==3%color images
[897]325            Aread=sum(double(Aread),3);% take the sum of color components
[454]326        end
[897]327        Ak(:,:,ifield)=Aread;
[24]328    end
[897]329    Asort=sort(Ak,3);%sort the luminosity of images at each point
330    B=Asort(:,:,rank);%background image
[1178]331
[897]332    %% substract the first background image to the first images
[1178]333    disp( 'first background image will be substracted')
[1124]334    for ifield=1:step*(halfnbaver+1)% nbre of images treated by the first background image
[897]335        Acor=double(Ak(:,:,ifield))-double(B);%substract background to the current image
336        Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
[1178]337        ifile=indselect(j_slice,ifield);
338        newname=fullfile_uvmat(OutputPath,OutputDir,RootFileOut,FileExtOut,NomTypeOut,i_indices(ifile),[],j_indices(ifile));
339
[897]340        %write result file
[1124]341        if ~isequal(Param.ActionInput.SaturationValue,0)
342            C=levels(Acor,Param.ActionInput.SaturationValue);
[950]343            imwrite(C,newname,'BitDepth',16); % save the new image
[897]344        else
[1178]345            if isequal(FileInfo.BitDepth,16)
[897]346                C=uint16(Acor);
347                imwrite(C,newname,'BitDepth',16); % save the new image
348            else
349                C=uint8(Acor);
350                imwrite(C,newname,'BitDepth',8); % save the new image
[442]351            end
[676]352        end
[1178]353        disp([newname ' written'])
[897]354    end
[1178]355
[897]356    %% repeat the operation on a sliding series of images
[1178]357    disp('sliding background image will be substracted')
[897]358    if nbfield_series > nbaver_ima
359        for ifield = step*(halfnbaver+1):step:nbfield_series-step*(halfnbaver+1)% ifield +iburst=index of the current processed image
[1178]360            %             update_waitbar(WaitbarHandle,ifield/nbfield_series)
[904]361            if  ~isempty(RUNHandle)&&~strcmp(get(RUNHandle,'BusyAction'),'queue')
[897]362                disp('program stopped by user')
363                return
[676]364            end
[897]365            if nbaver_ima>step
[1178]366                Ak(:,:,1:nbaver_ima-step)=Ak(:,:,1+step:nbaver_ima);% shift the current image series by one burst (step)
[897]367            end
368            %incorporate next burst in the current image series
369            for iburst=1:step
[1178]370                ifile=indselect(j_slice,ifield+iburst+step*halfnbaver);
371                if CheckRelabel
[1180]372                    [filename,FrameIndex]=index2filename(Param.FileSeries,i_indices(ifile),j_indices(ifile),NbField_j);
373                    filename=fullfile(RootPath,SubDir,filename);
[1178]374                else
375                    filename=fullfile_uvmat(RootPath,SubDir,RootFile,FileExt,NomType,i_indices(ifile),[],j_indices(ifile));
376                    FrameIndex=frame_index(ifile);
[897]377                end
[1178]378                Aread=read_image(filename,FileType,MovieObject,FrameIndex);
[1124]379                if ndims(Aread)==3%case of color images
[897]380                    Aread=sum(double(Aread),3);% take the sum of color components
381                end
382                Ak(:,:,nbaver_ima-step+iburst)=Aread;% fill the last burst of the current image series by the new image
383            end
384            Asort=sort(Ak,3);%sort the new current image series by luminosity
385            B=Asort(:,:,rank);%current background image
386            %substract the background for the current burst
387            for iburst=1:step
388                Acor=double(Ak(:,:,step*halfnbaver+iburst))-double(B); %the current image has been already read ans stored as index step*halfnbaver+iburst in the current series
389                Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
[1178]390                ifile=indselect(j_slice,ifield+iburst);
391                newname=fullfile_uvmat(OutputPath,OutputDir,RootFileOut,FileExtOut,NomTypeOut,i_indices(ifile),[],j_indices(ifile));
[897]392                %write result file
[1124]393                if ~isequal(Param.ActionInput.SaturationValue,0)
394                    C=levels(Acor,Param.ActionInput.SaturationValue);
[950]395                    imwrite(C,newname,'BitDepth',16); % save the new image
[676]396                else
[1178]397                    if isequal(FileInfo.BitDepth,16)
[897]398                        C=uint16(Acor);
399                        imwrite(C,newname,'BitDepth',16); % save the new image
400                    else
401                        C=uint8(Acor);
402                        imwrite(C,newname,'BitDepth',8); % save the new image
403                    end
[214]404                end
[1178]405                disp([newname ' written'])
[24]406            end
[676]407        end
[24]408    end
[1178]409
[897]410    %% substract the background from the last images
[1178]411    disp('last background image will be substracted')
[897]412    for  ifield=nbfield_series-step*halfnbaver+1:nbfield_series
413        Acor=double(Ak(:,:,ifield-nbfield_series+step*(2*halfnbaver+1)))-double(B);
414        Acor=(Acor>0).*Acor; % put to 0 the negative elements in Acor
[1178]415        ifile=indselect(j_slice,ifield);
416        newname=fullfile_uvmat(OutputPath,OutputDir,RootFileOut,FileExtOut,NomTypeOut,i_indices(ifile),[],j_indices(ifile));
[897]417        %write result file
[1124]418        if ~isequal(Param.ActionInput.SaturationValue,0)
419            C=levels(Acor,Param.ActionInput.SaturationValue);
[950]420            imwrite(C,newname,'BitDepth',16); % save the new image
[616]421        else
[1178]422            if isequal(FileInfo.BitDepth,16)
[897]423                C=uint16(Acor);
424                imwrite(C,newname,'BitDepth',16); % save the new image
425            else
426                C=uint8(Acor);
427                imwrite(C,newname,'BitDepth',8); % save the new image
428            end
[394]429        end
[1178]430        disp([newname ' written'])
[394]431    end
[24]432end
433
[1124]434function C=levels(A,Coeff)
[214]435
[1124]436% nblock_y=100;%2*Param.TransformInput.BlockSize;
437% nblock_x=100;%2*Param.TransformInput.BlockSize;
438% [npy,npx]=size(A);
439% [X,Y]=meshgrid(1:npx,1:npy);
440%
441% %Backg=zeros(size(A));
442% %Aflagmin=sparse(imregionalmin(A));%Amin=1 for local image minima
443% %Amin=A.*Aflagmin;%values of A at local minima
444% % local background: find all the local minima in image subblocks
445% if CheckSubmedian
446%     fctblock= inline('median(x(:))');
447%     Backg=blkproc(A,[nblock_y nblock_x],fctblock);% take the median in  blocks
448%     %B=imresize(Backg,size(A),'bilinear');% interpolate to the initial size image
449%     A=A-imresize(Backg,size(A),'bilinear');% substract background interpolated to the initial size image
450% end
451% fctblock= inline('mean(x(:))');
452% AMean=blkproc(A,[nblock_y nblock_x],fctblock);% take the mean in  blocks
453% fctblock= inline('var(x(:))');
454% AVar=blkproc(A,[nblock_y nblock_x],fctblock);% take the mean in  blocks
455% Avalue=AVar./AMean;% typical value of particle luminosity
456% Avalue=imresize(Avalue,size(A),'bilinear');% interpolate to the initial size image
457%C=uint16(1000*tanh(A./(Coeff*Avalue)));
458C=uint16(Coeff*tanh(A./Coeff));
459%------------------------------------------
460% adjust the number of images used for the sliding average
461function [nbaver_ima,nbaver,step_out]=adjust_slidinglength(nb_aver_in,step)
462%nbaver_ima=str2double(nb_aver_in);%number of images for the sliding background
463nbaver=ceil(nb_aver_in/step);%number of bursts for the sliding background
464if isequal(mod(nbaver,2),0)% if nbaver is even
465    nbaver=nbaver+1;%set the number of bursts to an odd number (so the middle burst is defined)
[951]466end
[1124]467step_out=step;
468if nbaver>1
469    nbaver_ima=nbaver*step;% correct the nbre of images corresponding to nbaver
470else
471    nbaver_ima=nb_aver_in;
472    if isequal(mod(nbaver_ima,2),0)% if nbaver_ima is even
473        nbaver_ima=nbaver_ima+1;%set the number of bursts to an odd number (so the middle burst is defined)
474    end
475    step_out=1;
476end
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